TY - JOUR
AU1 - Chaturvedi Misra, V.
AU2 - Pai B, Ganesh
AU3 - Tiwari, N.
AU4 - Patro, B. S.
AU5 - Ghorui, S.
AB - The tuning capacity of a novel excitation frequency controlled cold atmospheric pressure plasma jet in terms of reactive species density and its biological effect i.e., cell proliferation with changes in the excitation frequency was examined. The various reactive species produced were identified using optical emission spectroscopy. The physicochemical properties of the PTW (Plasma Treated Water) generated by the source were found to be almost linearly related with the time of treatment. For the presence of reactive species (H2O2, NO3− and NO2−), a comparison of plasma-treated water and cell culture medium at 11 kHz has been established. It has been observed that the variation in excitation frequency and treatment duration have important impact on cancer cell viability. In this regard, measuring the induced reactive species confirmed the strongest effect with CAP generated at 11 kHz frequency. Additionally, the 11 kHz frequency CAP produced the best biological outcome, among the different frequencies tested i.e., 11, 14, 22 and 26 kHz, as assessed by the reduction in proliferation of two different breast cancer cells (MCF-7 and MDA-MB-231). Further, the mechanistic aspects of CAP generated with 11 kHz frequency in inducing breast cancer cell death were investigated. The cancer cell death was rescued by co-treatment with N-acetyl cysteine, which indicated that the cell death effected by CAP is through the generation of reactive oxygen species. It has been shown that 11 kHz frequency CAP treatment induces excessive DNA damage and a prominent G1 phase cell cycle arrest and consequently cell death by apoptosis.
TI - Excitation Frequency Effect on Breast Cancer Cell Death by Atmospheric Pressure Cold Plasma
JF - Plasma Chemistry and Plasma Processing
DO - 10.1007/s11090-023-10312-3
DA - 2023-03-01
UR - https://www.deepdyve.com/lp/springer-journals/excitation-frequency-effect-on-breast-cancer-cell-death-by-atmospheric-TcvP0GkZg0
SP - 467
EP - 490
VL - 43
IS - 2
DP - DeepDyve
ER -