TY - JOUR
AU1 - Parker, Matthew F L
AU2 - López-Álvarez, Marina
AU3 - Alanizi, Aryn A
AU4 - Luu, Justin M
AU5 - Polvoy, Ilona
AU6 - Sorlin, Alexandre M
AU7 - Qin, Hecong
AU8 - Lee, Sanghee
AU9 - Rabbitt, Sarah J
AU1 - Pichardo-González, Priamo A
AU1 - Ordonez, Alvaro A
AU1 - Blecha, Joseph
AU1 - Rosenberg, Oren S
AU1 - Flavell, Robert R
AU1 - Engel, Joanne
AU1 - Jain, Sanjay K
AU1 - Ohliger, Michael A
AU1 - Wilson, David M
AB - BackgroundVertebral discitis-osteomyelitis (VDO) is a devastating infection of the spine that is challenging to distinguish from noninfectious mimics using computed tomography and magnetic resonance imaging. We and others have developed novel metabolism-targeted positron emission tomography (PET) radiotracers for detecting living Staphylococcus aureus and other bacteria in vivo, but their head-to-head performance in a well-validated VDO animal model has not been reported.MethodsWe compared the performance of several PET radiotracers in a rat model of VDO. [11C]PABA and [18F]FDS were assessed for their ability to distinguish S aureus, the most common non-tuberculous pathogen VDO, from Escherichia coli.ResultsIn the rat S aureus VDO model, [11C]PABA could detect as few as 103 bacteria and exhibited the highest signal-to-background ratio, with a 20-fold increased signal in VDO compared to uninfected tissues. In a proof-of-concept experiment, detection of bacterial infection and discrimination between S aureus and E coli was possible using a combination of [11C]PABA and [18F]FDS.ConclusionsOur work reveals that several bacteria-targeted PET radiotracers had sufficient signal to background in a rat model of S aureus VDO to be potentially clinically useful. [11C]PABA was the most promising tracer investigated and warrants further investigation in human VDO.
TI - Evaluating the Performance of Pathogen-Targeted Positron Emission Tomography Radiotracers in a Rat Model of Vertebral Discitis-Osteomyelitis
JF - Journal of Infectious Diseases
DO - 10.1093/infdis/jiad159
DA - 2023-10-03
UR - https://www.deepdyve.com/lp/oxford-university-press/evaluating-the-performance-of-pathogen-targeted-positron-emission-SsJwwZVMfe
SP - S281
EP - S290
VL - 228
IS - Supplement_4
DP - DeepDyve
ER -