TY - JOUR
AU - Strasburg, Gale M.
AB - Abstract The pharmacokinetic behavior of cyclopiazonic acid (CPA) was determined in market weight pigs using a competitive indirect ELISA developed for the determination of the mycotoxin in various biological matrices. Sample preparation for corn and skeletal muscle was achieved with a single extraction and recoveries of 53 ± 6% over the effective range of the standard curve. The detection limit of CPA was 1 ppb in plasma, which required no extraction, and 20 ppb in corn and skeletal muscle with average intra- and interassay CV of 11 and 23%, respectively. Levels of CPA contamination in corn grown and stored in Michigan were unremarkable compared with published toxicity thresholds; the highest level of CPA found in any sample was 47 ppb. In pigs given a 20-mg i.v. bolus, CPA distributed rapidly among three compartments, with an overall volume of distribution (49 L) nearly equivalent to total body water. Cyclopiazonic acid was eliminated with a half-life of 24 h. Estimates of these pharmacokinetic parameters were supported by the achievement of steady-state plasma CPA levels within 6 d in pigs consuming a diet containing 10 ppm CPA, and by measured concentrations of CPA in plasma (410 ± 44 ng/mL) and skeletal muscle (469 ± 86 ng/ g). From these and other data, we concluded that the threat of CPA toxicity to livestock from consumption of cereal grains or to humans from consumption of animal products is minimal. Copyright © 1999 by American Society of Animal Science
TI - Analysis and pharmacokinetics of cyclopiazonic acid in market weight pigs
JO - Journal of Animal Science
DO - 10.2527/1999.771173x
DA - 1999-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/analysis-and-pharmacokinetics-of-cyclopiazonic-acid-in-market-weight-Sk3mKUBGeo
SP - 173
EP - 179
VL - 77
IS - 1
DP - DeepDyve
ER -