TY - JOUR
AU - Weissman, S., M.
AB - Abstract Double stranded human globin cDNA was synthesized by use of viral reverse transcriptase from globin mRNA of cord blood of premature infants requiring exchange transfusions. The cDNA was introduced into plasmids and the recombinant DNA plasmids used to transform E. coli X1776. A number of transformants were obtained. Plasmid DNA from selected colonies was isolated and characterized for the type of globin cDNA it contained by three types of procedures: 1) hybridization to previously characterized 3 H-labeled α, β and γ cDNA; 2) analysis of the size and nature of fragments produced by digestion of the plasmid DNA by different restriction endonucleases; and 3) by rapid DNA sequence analysis of selected DNA fragments produced by restriction endonuclease digestion. Analysis by these techniques of plasmid DNA from different colonies has definitively identified the presence of human α, β or γ cDNA sequences in different plasmids. This content is only available as a PDF. © Information Retrieval Limited 1 Falconberg Court London W1V5FG England
TI - Insertion of synthetic copies of human globin genes into bacterial plasmids
JO - Nucleic Acids Research
DO - 10.1093/nar/5.2.563
DA - 1978-02-01
UR - https://www.deepdyve.com/lp/oxford-university-press/insertion-of-synthetic-copies-of-human-globin-genes-into-bacterial-ShAnFPf3O4
SP - 563
EP - 581
VL - 5
IS - 2
DP - DeepDyve
ER -