TY - JOUR
AU - Havran, W
AB - Purpose To investigate the role of a potential costimulatory molecule, CD100, on intraepithelial lymphocytes (IEL) function during murine colitis. Description of Project During the disease course of colitis, IEL bearing the γδ T-cell receptor become activated and secrete cytokines and growth factors including keratinocyte growth factor (KGF) that are important in repair of epithelial damage and down regulation of inflammation. Little is known about moleculesthat regulate γδ IEL function. Our lab has identified CD100 as a potential costimulatory molecule that can impact the activation of γδ T-cells in vitro. We investigated the role of CD100 on IEL function during colitis by administering dextran sulfate sodium (DSS) to CD100 null mice. Results and Conclusions Both αβ and γδIELs were found to express CD100, while enterocytes expressed a ligand to CD100, Plexin-B2. Upon DSS administration, colitis severity was found to be significantly worse in CD100 null mice as compared to wildtype mice, with increased weight loss, higher histological disease score, and increased length of lesions. The severe disease of the CD100 null mice was partly attributable to the failure of the colon epithelium and the resident IEL to increase proliferation in response to colitis. γδ IEL are known to mediate epithelial proliferation by expressing the potent mitogen KGF. γδ IEL from wild-type mice treated with DSS significantly increased their expression of KGF mRNA while γδ IEL from CD100 null mice failed to do so. In conclusion, CD100 and Plexin-B2 appearto be important in epithelial recovery from colitis by enhancing the function of γδ IEL. Supported by NIH grants A152257 and A107244-23 and a fellowship from the Crohn's and Colitis Foundation © 2007 Crohn's & Colitis Foundation of America, Inc.
TI - P-0012: CD100 modulation of intraepithelial lymphocyte function during colitis
JF - Inflammatory Bowel Diseases
DO - 10.1097/00054725-200705005-00032
DA - 2007-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/p-0012-cd100-modulation-of-intraepithelial-lymphocyte-function-during-Sf2aGvvH0S
SP - 652
EP - 652
VL - 13
IS - suppl_5
DP - DeepDyve
ER -