TY - JOUR
AU1 - van der Pol, Jens
AU2 - Machnik, Marc
AU3 - Biselli, Manfred
AU4 - Portela-Klein, Theresa
AU5 - de Gooijer, Cornelis
AU6 - Tramper, Johannes
AU7 - Wandrey, Christian
AB - The monoclonal-antibody production of an immobilized hybridoma cell line cultivated in a fluidized-bed reactor was monitored on-line for nearly 900 h. The monoclonal antibody concentration was determined by an immuno affinity-chromatography method (ABICAP). Antibodies directed against the product, e.g. IgG, were immobilized on a micro-porous gel and packed in small columns. After all IgG present in the sample was bound to the immobilized antibodies, unbound proteins were removed by rinsing the column. Elution of the bound antibodies followed and the antibodies were determined by fluorescence. The analytical procedure was automated with a robotic device to enable on-line measurements. The correlation between the on-line determined data and antibody concentrations measured by HPLC was linear.
TI - On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells
JF - Cytotechnology
DO - 10.1023/A:1007913128209
DA - 2004-10-15
UR - https://www.deepdyve.com/lp/springer-journals/on-line-immunoanalysis-of-monoclonal-antibodies-during-a-continuous-SeuW6CfT8A
SP - 19
EP - 30
VL - 24
IS - 1
DP - DeepDyve
ER -