TY - JOUR AU - Heinze, Katrin G AB - SummaryWe introduce software for reading, writing and processing fluorescence single-molecule and image spectroscopy data and developing analysis pipelines to unify various spectroscopic analysis tools. Our software can be used for processing multiple experiment types, e.g. for time-resolved single-molecule spectroscopy, laser scanning microscopy, fluorescence correlation spectroscopy and image correlation spectroscopy. The software is file format agnostic and processes multiple time-resolved data formats and outputs. Our software eliminates the need for data conversion and mitigates data archiving issues.Availability and implementationtttrlib is available via pip (https://pypi.org/project/tttrlib/) and bioconda while the open-source code is available via GitHub (https://github.com/fluorescence-tools/tttrlib). Presented examples and additional documentation demonstrating how to implement in vitro and live-cell image spectroscopy analysis are available at https://docs.peulen.xyz/tttrlib and https://zenodo.org/records/14002224. TI - tttrlib: modular software for integrating fluorescence spectroscopy, imaging, and molecular modeling JO - Bioinformatics DO - 10.1093/bioinformatics/btaf025 DA - 2025-01-21 UR - https://www.deepdyve.com/lp/oxford-university-press/tttrlib-modular-software-for-integrating-fluorescence-spectroscopy-SEaG4Mazdk VL - 41 IS - 2 DP - DeepDyve ER -