TY - JOUR
AU - Farrar, W. Edmund
AB - THE JOURNAL OF INFECTIOUS DISEASES • VOL. 148, NO.1. JULY 1983 © 1983 by The University of Chicago. All rights reserved. 0022-1899/83/4801-0001$00.75 MEDICAL PERSPECTIVE w. Edmund Farrar, Jr From the Infectious Diseases and Immunology Division, Department of Medicine, Medical University of South Carolina, Charleston, South Carolina Within the last few years some of the originally strands from different sources and may allow a arcane methods of molecular biology have been quantitative assessment of base sequence homolo­ simplified, and the molecular analysis of plasmids gy. (1) DNA-DNA hybridization in liquid phase is being applied very productively to the investiga­ may be used to determine the extent of homology tion of epidemiologic problems. Much of the work between two plasmid molecules [5, 6]. In this tech­ done so far has been in the realm of nosocomial nique, the DNA of one plasmid is radiolabeled, infections [l, 2], but a few studies have dealt with the double strands of both plasmids are separated problems of a broader nature. into single strands, anq the four strands are mixed together and allowed to reanneal. After reanneal­ ing, any single-stranded DNA remaining is re­ Molecular Techniques Useful in Plasmid Analysis moved with SI nuclease, and 
TI - Molecular Analysis of Plasmids in Epidemiologic Investigation
JO - The Journal of Infectious Diseases
DO - 10.1093/infdis/148.1.1
DA - 1983-07-01
UR - https://www.deepdyve.com/lp/oxford-university-press/molecular-analysis-of-plasmids-in-epidemiologic-investigation-SAoqB5V0fC
SP - 1
EP - 6
VL - 148
IS - 1
DP - DeepDyve
ER -