TY - JOUR
AU1 - Luo, J.-P.
AU2 - Jia,  J.-F.
AB - A reproducible release of viable protoplasts was obtained from friable calli of Astragalus adsurgens . Protoplasts underwent sustained divisions and formed cell colonies when cultured in either liquid or agarose-solidified Kao and Michayluk (1975) protoplast medium (KM8P) supplemented with 1.5 mg/l 2,4-D, 0.5 mg/l BA and 0.5 M glucose. Compared to liquid culture, agarose bead culture improved division frequency effectively, the two culture systems showing a plating efficiency of 0.8±0.5% and 6.5±0.7%, respectively. Upon transfer to Murashige and Skoog (1962) medium (MS) with 1–2 mg/l BA, alone or in combination with NAA or 2,4-D at 0.1 mg/l, the protoplast-derived calli produced complete plantlets through somatic embryogenesis. The maximum percentage of calli producing somatic embryos (52.5± 2.2%) occurred on MS medium containing 0.1 mg/l NAA and 1 mg/l BA, whereas the maximum number of calli regenerating plantlets (64.7±6.2) was obtained on MS medium with 0.1 mg/l NAA and 2 mg/l BA.
TI - Plant regeneration from callus protoplasts of the forage legume Astragalus adsurgens Pall.
JF - Plant Cell Reports
DO - 10.1007/s002990050399
DA - 1998-02-01
UR - https://www.deepdyve.com/lp/springer-journals/plant-regeneration-from-callus-protoplasts-of-the-forage-legume-Rn50V0kM4r
SP - 313
EP - 317
VL - 17
IS - 4
DP - DeepDyve
ER -