TY - JOUR AU1 - Lucena,, E. AU2 - Lucena,, C. AU3 - Gómez,, M. AU4 - Ortiz,, J.A. AU5 - Ruiz,, J. AU6 - Arango,, A. AU7 - Diaz,, C. AU8 - Beuerman,, C. AB - Abstract Sperm washing techniques, based on the swim-up principle used before inseminating the human oocyte in in-vitro fertilization and embryo transfer programmes (IVF–ET), usually require prior centrifugation which causes damage to the sperm cell. A technique is described for separating sperm at laboratory temperature based on sperm migration–sedimentation principles, using two concentric tubes and recovering 70–90% forward-moving cells. A group of 17 patients is presented who were managed with this method. The results were 85% fertilization rate, 4% polyspermia and six clinical pregnancies spermatozoa, migration-sedimentation, swim-up This content is only available as a PDF. © IRL Press at Oxford University Press TI - Recovery of motile sperm using the migration–sedimentation technique in an in-vitro fertilization - embryo transfer programme JF - Human Reproduction DO - 10.1093/oxfordjournals.humrep.a136864 DA - 1989-02-01 UR - https://www.deepdyve.com/lp/oxford-university-press/recovery-of-motile-sperm-using-the-migration-sedimentation-technique-Rge5OPTPRM SP - 163 EP - 165 VL - 4 IS - 2 DP - DeepDyve ER -