TY - JOUR
AU - Burk, Martyn W.
AB - Two monoclonal antibodies produced by hybridomas that define cell surface antigens expressed on cultured human melanoma cells were identified by an indirect 125-protein A binding assay. These antigens were not detectable on autologous or allogeneic lymphoblastoid cells. The first antibody (an lgG2bimmunoglobulin) bound to 17 of 17 melanoma lines, 12 of 14 carcinomas and sarcomas, 2 of 2 fetal cell lines, 0 of 13 lymphoblastoid cell lines, and weakly to 3 of 7 leukemia lines. The second monoclonal antibody (IgG1) reacted with 13 of 17 melanomas, 3 of 14 other solid tumor cell lines, and weakly with the fetal cells, but not with the leukemic or lymphoblastoid cell lines. Quantitative absorption of these two monoclonal antibodies confirmed that the antigens were expressed on the melanoma cells but were absent from autologous B-cell lines derived from the same cancer patient. Immunoprecipi-tation of lactoperoxidase 125I-labeled membrane antigens from the melanoma cells followed by polyacrylamide gel electrophoresis identified a 95K protein complexed with the IgG2bantibody and a 120K protein bound to the IgG1antibody.
TI - Monoclonal Antibodies to Human Melanoma
JF - Journal of Biological Response Modifiers
DA - 1982-01-01
UR - https://www.deepdyve.com/lp/wolters-kluwer-health/monoclonal-antibodies-to-human-melanoma-RepOZxf9Gr
VL - 1
IS - 1
DP - DeepDyve
ER -