TY - JOUR
AU - Michel, Laurence
AB - Introduction: Transforming growth factor (TGF)-β and interleukin (IL)-13 play a crucial role in the pathogenesis of systemic sclerosis (SSc), partly through activation of collagen production that leads to fibrosis. The aim of the present study was to determine whether TFG-β alters IL-13 production in T lymphocytes from patients with SSc from that seen in those of healthy donors. Methods: IL-13 mRNA and protein synthesis under TFG-β exposure was measured in circulating T lymphocytes from healthy donors and patients with SSc and also in the Jurkat Th2 T-cell line, using quantitative real-time PCR and fluorescence-activated cell sorting analysis, respectively. The involvement of Smad and GATA-3 transcription factors was assessed by using specific inhibitors and small interfering RNA, and the binding capacity of GATA-3 to the IL-13 gene promoter was evaluated by chromatin immunoprecipitation assay. Results: TGF-β induced a significant decrease in IL-13 mRNA and protein levels in lymphocytes from healthy donors (mean [±SD] inhibition of 30 % ± 10 % and 20 % ± 7 %, respectively; p<0.05).Incontrast,TGF-β promoted a significant + + increase in IL-13 mRNA levels and IL-13 synthesis by CD4 and CD8 T-cell subtypes from patients with SSc, with respective increases of 2.4 ± 0.3-fold, 1.6 ± 
TI - Transforming growth factor-β increases interleukin-13 synthesis via GATA-3 transcription factor in T-lymphocytes from patients with systemic sclerosis
JF - Arthritis Research & Therapy
DO - 10.1186/s13075-015-0708-0
DA - 2015-07-31
UR - https://www.deepdyve.com/lp/springer-journals/transforming-growth-factor-increases-interleukin-13-synthesis-via-gata-RVz6j0BHSM
SP - 1
EP - 15
VL - 17
IS - 1
DP - DeepDyve
ER -