TY - JOUR
AU1 - Tian, Xingfang
AU2 - Zhu, Xiaoce
AU3 - Wang, Meng
AU4 - Guo, Tingting
AU5 - Kong, Jian
AB - Lactobacillus plantarum is a catalase-negative species and distributes in human intestinal tracts. However, the cytoprotective effects of the catalase-activated L. plantarum strain have yet to be exploited against reactive oxygen species (ROS). Here, a catalase-activated L. plantarum CGMCC 6888 (CatA+) was obtained using exogenous added heme. The scavenging free radical abilities of this strain were obviously increased. Moreover, the activated catalase A in L. plantarum CGMCC 6888 endowed the intestinal epithelium NCM460 with lower ROS content after degrading H2O2. In addition, the transcription levels of Nrf2 and Nrf2-related antioxidant enzyme genes (HO-1, GCLC, NQO-1 and TXNRD1) and tight junction protein genes (ZO-1, OCLN, and JAM-1) were upregulated significantly when co-incubated with CGMCC 6888/CatA+. This work confirmed that the catalase A conferred L. plantarum with the strong protection effects in the intestinal epithelial cells against ROS.
TI - Stimulation of Heme-Dependent Catalase Enhanced the Cytoprotective Effect of Lactobacillus plantarum against Oxidative Stress
JF - Applied Microbiology
DO - 10.3390/applmicrobiol3010011
DA - 2023-01-23
UR - https://www.deepdyve.com/lp/multidisciplinary-digital-publishing-institute/stimulation-of-heme-dependent-catalase-enhanced-the-cytoprotective-RSh36YYb8d
SP - 131
EP - 144
VL - 3
IS - 1
DP - DeepDyve
ER -