TY - JOUR AU - Patil, Nayana AB - [With the advent of DNA manipulation techniques, it is possible to generate identical copies of desired genes. These copies are introduced into a suitable vector for proliferation and can also be modified in vitro, altering the expression and function to obtain the required product. These cloning methods undergo advancements continuously and therefore various strategies have been designed to transfer sequences of interest efficiently. Recombinant DNA technology (RDT) has also enabled researchers to recognize the utility of genes in various fields of basic research, agriculture, and biomedical engineering. Here we discuss different cloning strategies that are established to successfully carry out gene transfer.] TI - A Complete Guide to Gene Cloning: From Basic to Advanced : Overview of Gene Cloning Strategies DA - 2022-04-27 UR - https://www.deepdyve.com/lp/springer-journals/a-complete-guide-to-gene-cloning-from-basic-to-advanced-overview-of-RSOhFDsxSA DP - DeepDyve ER -