TY - JOUR
AU - Hermann, Gilles
AB - The objective of our work is to develop a tool for automatic analysis of 2D membrane protein crystal images in Transmission Electron Microscopy (TEM). The success of crystallization experiments is evaluated at high magnification. The crystalline structure of a membrane can be observed when no other membranes are superposed. It is therefore necessary to identify mono-layer membranes. In this paper we introduce an algorithm that determines the stacking-level of membranes. Our method determines a quantum, a gray-level quantity that is characteristic of a non-stacked membrane. In this way we are able to label each region qualitatively and construct a stacking-level map that distinguishes from non-stacked to up to four-level stacked membranes. This map provides the regions that will trigger a new image acquisition at higher magnification.
TI - Evaluation of membrane stacking in electron microscope images
JF - Proceedings of SPIE
DO - 10.1117/12.807268
DA - 2009-02-05
UR - https://www.deepdyve.com/lp/spie/evaluation-of-membrane-stacking-in-electron-microscope-images-RCM1CcqPpp
SP - 725110
EP - 725110-11
VL - 7251
IS - 1
DP - DeepDyve
ER -