TY - JOUR
AU - NOZAKI,, Mitsuhiro
AB - Abstract From a comparison of the gel chromatographic properties of large randomly-coiled polypeptides in 6 M guanidine hydrochloride and of large globular proteins, we found that the distribution coefficient was more closely correlated with the intrinsic viscosity-based Stokes radius than with the translational frictional coefficient-based Stokes radius. This means that the effect of the hydrodynamic flow of dissolved molecules during gel chromatography should be considered. The ratio of transport of solute by bulk flow as compared with that by net diffusion (i.e., Brownian motion) is large under some conditions. On the other hand, we consider that the distribution coefficient obtained in static equilibrium experiments should be determined by the translational frictional coefficient-based Stokes radius, since the solvent does not flow. On this basis, we discuss the meaning of the Stokes radius and the separation mechanism of macromolecules by gel filtration. This content is only available as a PDF. Author notes 1 This research was supported in part by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan to M.N. © THE JOURNAL OF BIOCHEMISTRY
TI - Interpretation of the Stokes Radius of Macromolecules Determined by Gel Filtration Chromatography
JO - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a134183
DA - 1983-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/interpretation-of-the-stokes-radius-of-macromolecules-determined-by-R0sGxwP0TN
SP - 99
EP - 106
VL - 93
IS - 1
DP - DeepDyve
ER -