TY - JOUR AU - Bissel, E, R AB - Abstract The first commercially available centrifugal analyzer having fluorescence detection capability was used to develop kinetic fluorometric assays for several proteinases. The substrates were all synthetic oligopeptides incorporating 7-amino-4-trifluoromethylcoumarin, a molecule that can be detected either spectrophotometrically or fluorometrically. We thus compared the centrifugal analyzer spectrophotometric and fluorometric detection systems, finding fluorescence detection to be 50-fold more sensitive. We also compared the sensitivity of the fluorescence detector to that of a conventional spectrofluorometer by determining the minimum detection limit for each enzyme on both instruments; we found them to be similar in sensitivity. As an illustrative application, we measured the cathepsin B-like activity in serum samples from 55 women. The median enzyme activity of women taking oral contraceptives and pregnant women was increased two- and threefold, respectively, over the control group (about 5% CV within run, and 10% CV between runs). This content is only available as a PDF. © 1981 The American Association of Clinical Chemistry This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) TI - Automated enzyme assays by use of a centrifugal analyzer with fluorescence detection. JF - Clinical Chemistry DO - 10.1093/clinchem/27.2.256 DA - 1981-02-01 UR - https://www.deepdyve.com/lp/oxford-university-press/automated-enzyme-assays-by-use-of-a-centrifugal-analyzer-with-Qvrf21ynbU SP - 256 EP - 262 VL - 27 IS - 2 DP - DeepDyve ER -