TY - JOUR
AU - Godfrey,, D.G.
AB - Abstract 867 individuals from 3 sites near the town of Adjumani in the East Moyo region of north-west Uganda were investigated clinically and serologically for evidence of current trypanosome infections. Blood samples were taken from 94 persons with a positive card agglutination test for trypanosomiasis (catt) and clinical suspects and inoculated into the kit for in vitro isolation of Trypanosoma brucei gambiense (kivi). Amongst this group, 30 parasitaemic individuals were identified by microhaematocrit centrifugation and the quantitative buffy coat technique (QBC®). Only 80% of these isolates, and one isolate from an aparasitaemic individual, grew in culture. The success or failure of cultures from parasitaemic patients was unrelated to the size of the trypanosome inoculum. The implications of these results and possible reasons for the failure of kivi are discussed. trypanosomiasis, Trypanosoma brucei gambiense, isolation in vitro, kivi, Uganda References Aerts et al., 1992 Aerts D. , Truc P. , Penchenier L. , Claes Y. , Le Ray D. . A kit for in vitro isolation of trypanosomes in the field: first trial with sleeping sickness patients in the Congo Republic , Transactions of the Royal Society of Tropical Medicine and Hygiene , 1992 , vol. 86 (pg. 394 - 395 ) Google Scholar Crossref Search ADS PubMed WorldCat Bailey and Smith, 1992 Bailey J.W. , Smith D.H. . The use of the acridine orange QBC® technique in the diagnosis of African trypanosomiasis , Transactions of the Royal Society of Tropical Medicine and Hygiene , 1992 , vol. 86 pg. 630 Google Scholar Crossref Search ADS PubMed WorldCat Bailey and Smith, 1994 Bailey J.W. , Smith D.H. . The quantitative buffy coat for the diagnosis of trypanosomes , Tropical Doctor , 1994 , vol. 24 (pg. 54 - 56 ) Google Scholar PubMed OpenURL Placeholder Text WorldCat Dukes et al., 1991 Dukes P. , McNamara J.J. , Godfrey D.G. . Elusive trypanosomes , Annals of Tropical Medicine and Parasitology , 1991 , vol. 83 (pg. 21 - 32 ) OpenURL Placeholder Text WorldCat Enyaru et al., 1993 Enyaru J.C.K. , Allingham R. , Bromidge T. , Kanmogne G.D. , Carasco J.F. . The isolation and genetic heterogeneity of Trypanosoma brucei gambiense from north-west Uganda , Acta Tropica , 1993 , vol. 54 (pg. 31 - 39 ) Google Scholar Crossref Search ADS PubMed WorldCat Kuzoe, 1993 Kuzoe F.A.S. . Current situation of African trypanosomiasis , Acta Tropica , 1993 , vol. 54 (pg. 153 - 162 ) Google Scholar Crossref Search ADS PubMed WorldCat Lanham, 1977 Lanham S.M. . Protozoological methods in the field diagnosis of trypanosomiasis , Transactions of the Royal Society of Tropical Medicine and Hygiene , 1977 , vol. 71 (pg. 8 - 10 ) Google Scholar Crossref Search ADS PubMed WorldCat Lumsden et al., 1979 Lumsden W.H.R. , Kimber C.D. , Evans D.A. , Doig S.J. . Trypanosoma brucei: miniature anion-exchange centrifugation technique for detection of low parasitaemias: adaptation for field use , Transactions of the Royal Society of Tropical Medicine and Hygiene , 1979 , vol. 73 (pg. 312 - 317 ) Google Scholar Crossref Search ADS PubMed WorldCat Truc et al., 1992 Truc P. , Aerts D. , McNamara J.J. , Claes Y. , Allingham R. , Le Ray D. , Godfrey D.G. . Direct isolation in vitro of Trypanosoma brucei from man and other animals, and its potential value for the diagnosis of gambian trypanosomiasis , Transactions of the Royal Society of Tropical Medicine and Hygiene , 1992 , vol. 86 (pg. 627 - 629 ) Google Scholar Crossref Search ADS PubMed WorldCat Truc et al., 1994 Truc P. , Bailey J.W. , Doua F. , Laveissière C. , Godfrey D.G. . A comparison of parasitological methods for the diagnosis of gambian trypanosomiasis in an area of low endemicity in Côte d'Ivoire , Transactions of the Royal Society of Tropical Medicine and Hygiene , 1994 , vol. 88 (pg. 419 - 421 ) Google Scholar Crossref Search ADS PubMed WorldCat Vickerman, 1965 Vickerman K. . Polymorphism and mitochondrial activity in sleeping sickness trypanosomes , Nature , 1965 , vol. 20 (pg. 762 - 766 ) Google Scholar Crossref Search ADS WorldCat Vickerman et al., 1988 Vickerman K. , Tetley L. , Hendry K.A.H. , Turner M.R. . Biology of African trypanosomes in the tsetse fly , Biology of the Cell , 1988 , vol. 64 (pg. 109 - 119 ) Google Scholar Crossref Search ADS PubMed WorldCat Woo, 1970 Woo P.T.K. . The haematocrit centrifuge technique for the diagnosis of African trypanosomiasis , Acta Tropica , 1970 , vol. 27 (pg. 384 - 386 ) Google Scholar PubMed OpenURL Placeholder Text WorldCat This content is only available as a PDF. Author notes Diagnosis
TI - Isolation of Trypanosoma brucei gambiense from northern Uganda: evaluation of the kit for in vitro isolation (KIVI) in an epidemic focus
JF - Transactions of The Royal Society of Tropical Medicine and Hygiene
DO - 10.1016/0035-9203(95)90021-7
DA - 1995-08-01
UR - https://www.deepdyve.com/lp/oxford-university-press/isolation-of-trypanosoma-brucei-gambiense-from-northern-uganda-QuHg2riojp
SP - 388
EP - 389
VL - 89
IS - 4
DP - DeepDyve
ER -