TY - JOUR
AU - Brown, Ruby, W.
AB - Abstract The effect of epidermal growth factor (EGF) and fibroblast feeder layers on the proliferation of human tumor cells in soft agar was examined. The addition of EGF to medium supporting growth of tumor cells significantly increased (P≤0.02) the number of colonies grown from the cells of 36 of 58 patients (62%) with a variety of neoplasms. The increase in the number of colonies was dependent on the concentration of EGF and was maximal at a concentration of 50 ng EGF/ml. The addition of glucocorticoids did not potentiate the effect of EGF. Lethally irradiated fibroblast feeder layers alone only slightly increased the number of colonies. However, the addition of fibroblasts to cultures significantly increased the mitogenic effect of EGF. The results suggest that a proportion of epithelial-derived tumors retain responsiveness in vitro to physiologic growth regulators such as EGF. 2 Supported in part by Public Health Service grants CA26008 and CA28669 from the National Cancer Institute. 3 Research procedures were in accord with the ethical standards of the American Type Culture Collection. This content is only available as a PDF. Author notes 4 Cell Culture Department, American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852. 5 The authors thank Dr. Jack Killen, Dr. Juan Posada, and Dr. Philip Schein, George University School of Medicine, and Dr. Charles Currier, Washington Hospital Center, for providing the specimens used in these studies. We also thank Mrs. Shirley Mazur for preparation of the manuscript.
TI - Effect of Epidermal Growth Factor on Proliferation of Human Tumor Cells in Soft Agar
JF - JNCI: Journal of the National Cancer Institute
DO - 10.1093/jnci/67.4.825
DA - 1981-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/effect-of-epidermal-growth-factor-on-proliferation-of-human-tumor-QGlRcdWPD7
SP - 825
EP - 830
VL - 67
IS - 4
DP - DeepDyve
ER -