TY - JOUR AU - Zhu, Shuifang AB - This study characterized the viral small interfering RNAs (vsiRNAs) from Nicotiana tabacum cv. Samsun infected with a cucumber mosaic virus (CMV) 2b-deficient mutant. Most vsiRNAs were 21 -22 nucleotides in length and the 5′-terminal ends were dominated by A and U, respectively. The observed vsiRNAs were heterogeneously distributed throughout the CMV genome; however, most of the vsiRNAs were derived from sense strands, as opposed to antisense strands. These results demonstrate the conserved and specific function of Dicer-like (DCL), Argonaute (AGO) and RNA-dependent RNA polymerase (RDR) proteins in tobacco. Finally, it was revealed that vsiRNAs target abundant host genes, indicating complex roles for CMV vsiRNAs during the development of symptoms. TI - Characterization of siRNAs derived from cucumber mosaic virus in infected tobacco plants JO - Archives of Virology DO - 10.1007/s00705-017-3335-z DA - 2017-03-27 UR - https://www.deepdyve.com/lp/springer-journals/characterization-of-sirnas-derived-from-cucumber-mosaic-virus-in-O7UyoYHBbu SP - 2077 EP - 2082 VL - 162 IS - 7 DP - DeepDyve ER -