TY - JOUR AU1 - Reagan, Reginald L. AU2 - Hickman, Jean W. AU3 - Lillie, Mary G. AU4 - Brueckner, A. L. AB - MORPHOLOGICAL OBSERVATIONS BY ELECTRON MICROSCOPY OF THE HAMSTER-ADAPTED AND THE MOUSE-ADAPTED NEWCASTLE VIRUS AFTER CULTURE IN CHICK EMBRYOS REGINALD L. REAGAN, JEAN W. HICKMAN, MARY G. LILLIE, AND A. L. BRUECKNER From the Maryland State Board of Agriculture, Live Stock Sanitary Service Laboratory, University of Maryland, College Park, Maryland Micrographs of purified egg-adapted of the same age when the same route of Newcastle virus suspended in 0.07 to injection was employed. One hundred cc 0.15 M sodium chloride by Bang--" of the pooled allantoic fluid was centri­ showed that tailed or sperm-like forms fuged in a horizontal centrifuge for 15 minutes at 1000 r.p.m, and then for 2 predominated. Cunha et al reported similar morphological characteristics. hours at 50,000 r.p.m. in a Spinco Ultra Centrifuge. The sediment was resus­ Reagen et al also found sperm-like forms in the hamster-adapted New­ pended with saline, placed on parolodion castle virus, California strain 11914, prepared screens, shadowed with chro­ when the virus particles were suspended mium at arc tan 1/6, and examined in normal saline. under the RCA Emu electron micro­ scope. Small tailed bodies and a few Hamster-adapted Newcastle virus of the 249th intracerebral passage was cul­ nontailed forms were observed TI - Morphological Observations by Electron Microscopy of the Hamster-Adapted and the Mouse-Adapted Newcastle Virus after Culture in Chick Embryos JF - The Journal of Infectious Diseases DO - 10.1093/infdis/85.3.256 DA - 1949-11-01 UR - https://www.deepdyve.com/lp/oxford-university-press/morphological-observations-by-electron-microscopy-of-the-hamster-NPBEsvT0us SP - 256 EP - 258 VL - 85 IS - 3 DP - DeepDyve ER -