TY - JOUR AU - Sachar, E, J AB - Abstract We describe an improved competitive protein-binding radioassay for total plasma cortisol, which permits rapid analysis of many samples. Plasma, 0.1 mL, is directly applied to a Whatman 3MM filter-paper strip, which is extracted once with dichloromethane. The extract is evaporated and the residue is assayed directly by the micro-method of Murphy (J. Clin. Endocrinol. Metab. 27: 973, 1967). Comparison with the original Murphy method (ethanol extraction) yielded excellent agreement (r = 0.988). One hundred samples, in duplicate, can easily be assayed at a time. This content is only available as a PDF. © 1980 The American Association of Clinical Chemists, Inc. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) TI - Improved protein-binding radioassay for plasma cortisol. JF - Clinical Chemistry DO - 10.1093/clinchem/26.2.312 DA - 1980-02-01 UR - https://www.deepdyve.com/lp/oxford-university-press/improved-protein-binding-radioassay-for-plasma-cortisol-NAZv0s099k SP - 312 EP - 313 VL - 26 IS - 2 DP - DeepDyve ER -