TY - JOUR
AU1 - Cho, Su-Hyeon
AU2 - Kim, Song Rae
AU3 - Jeong, Myeong Seon
AU4 - Choi, Miri
AU5 - Park, SeonJu
AU6 - Kim, Kil-Nam
AB - ObjectiveTo demonstrate the anti-inflammatory activity of Brassica napus L. hydrosols (BNH) in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells.MethodsComposition analysis of BNH was conducted via gas chromatography-mass spectrometry after BNH were extracted. The nitric oxide (NO) production was measured using the Griess assay. Prostaglandin E2 (PGE2) production was evaluated with enzyme-linked immunosorbent assay. The effects of BNH on LPS-induced pro-inflammatory enzymes including inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) were evaluated using Western blot analysis. Furthermore, phosphorylation of nuclear factor-kappa B (NF-κB) and nuclear translocation of NF-κB p65 were evaluated with Western blot analysis and immunofluorescence staining, respectively.ResultsCompared with LPS-stimulated cells, BNH markedly decreased the generation of NO and PGE2 in LPS-stimulated RAW 264.7 cells (P<0.01 or P<0.05). Moreover, BNH inhibited protein levels of iNOS and COX-2 (P<0.01). Phosphorylation of NF-κB and nuclear translocation of NF-κB p65 was significantly inhibited by BNH (P<0.01 or P<0.05).ConclusionThe anti-inflammatory activities of BNH were mediated via blockage of the NF-κB signaling pathways in LPS-stimulated RAW 264.7 cells.
TI - Protective Effect of Brassica napus L. Hydrosols against Inflammation Response in RAW 264.7 Cells
JF - Chinese Journal of Integrative Medicine
DO - 10.1007/s11655-021-3330-9
DA - 2021-03-24
UR - https://www.deepdyve.com/lp/springer-journals/protective-effect-of-brassica-napus-l-hydrosols-against-inflammation-N9vFi84p09
SP - 273
EP - 279
VL - 27
IS - 4
DP - DeepDyve
ER -