TY - JOUR
AU1 - BADUR, S
AU2 - GRANGEOT-KEROS, L
AU3 - PILLOT, J
AB - SUMMARY In order to define the optimal conditions for detection of microbial antigens in urine, urinary HBsAg excreted during hepatitis B was chosen as a model. Using commercial kits, which mainly involve anti-discontinuous epitopes, we found urinary HBsAg in only 50% of patients with HBsAg in their sera. In contrast, with an inhibition method involving a monoclonal antibody recognizing a continuous epitope, urinary HBsAg was found in 100% of these patients. Structural analysis of HBsAg showed that urinary HBsAg is denatured; it can escape detection by commercial kits well fitted for detection of native serum HBsAg. General implications for the revelation of urinary microbial antigens are discussed. urinary, HBsAg, antigenuria, microbial, antigen degradation REFERENCES 1 Coonrod JD. Physical and immunologic properties of pneumococcal capsular polysaccharide produced during human infection . J Immunol 1974 ; 112 : 2193 – 201 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 2 Lebrun L , Guibert M, Pillot J. Failure of tests to detect pneumococcal antigens in urine . Eur J Clin Microbiol Infect Dis 1988 ; 7 : 83 . Google Scholar Crossref Search ADS PubMed WorldCat 3 Villarejos VM , Visna KA, Grutissez J, Rodriguez A. Role of saliva, urine and feces in the transmission of type B hepatitis . New Engl J Med 1974 ; 291 : 1375 – 7 . Google Scholar Crossref Search ADS PubMed WorldCat 4 Vittal SBW , Dourdourekas D, Steingmann F. Hepatitis B antigen in saliva, urine and tears . Am J Gastroenterol 1974 ; 61 : 133 – 5 . Google Scholar PubMed OpenURL Placeholder Text WorldCat 5 Kaiser L , Thomas JK, Patterson MJ, Sanchez TV, Shapiro RS, Mayor GH. Hepatitis B surface antigen in urine of renal transplant recipients . Ann Int Med 1981 ; 94 : 783 – 4 . Google Scholar Crossref Search ADS PubMed WorldCat 6 Neurath AR , Kent SBH, Stride N, Taylor P, Stevens CE. Hepatitis B virus containing pre S gene encoded domains . Nature 1985 ; 315 : 154 – 6 . Google Scholar Crossref Search ADS PubMed WorldCat 7 Petit MA , Maillard P, Capel F, Pillot J. Immunochcmical structure of the hepatitis B surface antigen vaccine. II. Analysis of antibody responses in human sera against the envelope proteins . Mol Immunol 1986 ; 23 : 511 – 23 . Google Scholar Crossref Search ADS PubMed WorldCat 8 Pillot J , Riottot MM, Geneste C, Phalente L, Mangalo R. Analysis of the vaccinating group specificity α of HBsAg with monoclonal antibodies. Identification of discontinuous epitopes . Develop Biol Standard 1984 ; 57 : 299 – 304 . Google Scholar OpenURL Placeholder Text WorldCat 9 Pillot J , Petit MA. Immunochemical structure of the hepatitis B surface antigen vaccine. I. Treatment of immobilized HBsAg by dissociation agents with or without enzymatic digestion and identification of polypeptides by protein blotting . Mol Immunol 1984 ; 21 : 53 – 60 . Google Scholar Crossref Search ADS PubMed WorldCat 10 Nilsson OB , Svalender PC, Larsson A. Immunization of mice and rabbits by intrasplenic deposition of nanogram quantities of protein attached to sepharose beads or nitrocellulose paper strips . J Immunol Meth 1987 ; 99 : 67 – 75 . Google Scholar Crossref Search ADS WorldCat 11 Towbin A , Staehelin T, Gordon J. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications . Proc Natl Acad Sci USA 1979 ; 76 : 4350 – 4 . Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - HBsAg in urine: a new approach for the detection of urinary antigens
JF - Clinical & Experimental Immunology
DO - 10.1111/j.1365-2249.1992.tb02991.x
DA - 2008-06-28
UR - https://www.deepdyve.com/lp/oxford-university-press/hbsag-in-urine-a-new-approach-for-the-detection-of-urinary-antigens-N0b9cLnGuN
SP - 298
EP - 303
VL - 87
IS - 2
DP - DeepDyve
ER -