TY - JOUR
AU - Seale, Madeleine
AB - In a world surrounded by a vast expanse of gaseous air, water is constantly evaporating from aqueous sources to their surroundings. This is a major problem for microscopic cells containing water essential for life. The genetic and chemical components that help to prevent water loss by creating a waxy cuticle around cells and tissues have now been described for a wide range of plant lineages in results published in this issue of Plant Physiology (Kong et al., 2020). Cuticle is found on the outer surface of the tissues of many land plants. It provides a barrier between the cells and the outside world preventing dehydration, impeding pathogen penetration, and protecting against UV radiation (Shepherd and Wynne Griffiths, 2006; Yeats and Rose, 2013). In some plants, cuticles have become highly specialized, leading to superhydrophobicity, which enables self-cleaning properties, or have fine-grained patterning that allows structural color to occur when light refracts off the surface (Barthlott et al., 2017). Many plants defective in cuticle formation also show fasciation, indicating that the cuticle can function to separate developing organs from one another and prevent fusion (Nawrath, 2002; Renault et al., 2017). The plant cuticle is typically composed of two major components: a complex of fatty acid polymers and phenolics called cutin that forms a framework, and wax components that surround the cutin scaffold (Fich et al., 2016). Cutin largely comprises 16- or 18-carbon fatty acids that have been oxygenated and hydroxylated. Other components are also found in smaller quantities, such as unsubstituted fatty acids, dicarboxylic acids, and phenolics, including ferulic acid and coumaric acid. These polymers and components may be cross-linked together and are thought to form an open, branched structure into and onto which waxes are deposited (Yeats and Rose, 2013; Fich et al., 2016). The wax component of cuticles generally consists of 20- to 34-carbon very-long-chain fatty acids. These often form a layer over the surface of cutin and can crystallize to create rough surface structures (Yeats and Rose, 2013). Kong et al. (2020) investigated the prevalence of cuticle biosynthesis genes across a range of both algae and land plants and revealed the composition and hydrophobic properties of cuticles in 13 representative land plants. They found that cuticle biosynthesis likely originated in the last common ancestor of land plants. While some cuticle biosynthesis genes were found in algae, particularly in the closest relatives to land plants, the Zygnematophyceae, no algal species that they examined contained a full set of the genes that would be required to make functional cuticle. In contrast, representatives of all necessary genes for cuticle biosynthesis were found in all land plants examined as well as genes required for the transport and secretion of cuticle components. Many of the gene families were more sparsely represented in bryophytes and had progressively expanded in gene number from lycophytes to monilophytes and seed plants. The presence of these genes does not necessarily imply a conserved function, so Kong et al. (2020) examined gene expression networks and established that many are coexpressed. They analyzed published genome annotations and transcriptomes of seven land plant species, including angiosperms, a lycophyte, and a bryophyte. As many cuticle biosynthesis, transport, and related regulatory genes were coexpressed, they surmised that there is likely to be a functional relationship between the genes. The coexpression across taxa also suggests that these genetic networks are at least partly conserved among land plants. As the number of genes increased across different plant lineages, so did the quantity of cuticle, which also changed in composition. The liverwort and hornwort that were examined had minimal cuticle that consisted largely of phenolic compounds in the cutin fraction and very-long-chain fatty acids for the cuticular wax components. In contrast, seed plants contained two to three times more cuticle than the liverwort and hornwort. Seed plant cutin contained large proportions of dicarboxylic acids and dihydroxy and trihydroxy acids with minimal phenolics. Waxes in seed plants were primarily very-long-chain alkanes and long (greater than C28) lipophilic compounds. Lycophytes and monilophytes had intermediate levels between bryophytes and seed plants of cuticle and the various components examined. Interestingly, the moss Physcomitrella/Physcomitrium patens was in many respects more similar to lycophytes than to the other bryophytes. This indicates that selective pressures to evolve specific types of cuticle may have acted in different ways within phylogenetic clades. Finally, Kong et al. (2020) also established that hydrophobicity and moisture retention are greater in seed plants than in the other lineages and lowest in bryophytes. They examined the contact angle of water droplets on phyllid or leaf surfaces, which is an indication of hydrophobicity: large angles (as in angiosperms) indicate a rounded droplet that is more repelled from the leaf surface, while smaller angles (as in bryophytes) indicate a hydrophilic surface that attracts water molecules more readily. Furthermore, these measurements correlated with the amount of water lost from plant tissues after being placed in a desiccating environment. These findings illustrate two critical features of cuticle formation. First, cuticle biosynthesis originated around the time of the origin of land plants, as all embryophytes examined exhibited comprehensive and conserved genetic networks likely to be capable of synthesizing cuticle. Additionally, there is little evidence of fully functional cuticle synthesis in algae. The early evolution of cuticle is supported by the presence of cuticle in 400-million-year-old plant fossils (Niklas, 1976). Kong et al. (2020) suggest that the cuticle synthesis machinery was gradually acquired in algae with the necessary components present in land plants. These gene families then expanded and were built upon during the evolution of land plant lineages (Fig. 1). Figure 1. Open in new tabDownload slide Schematic illustrating the proposed origins, evolutions, and characteristics of cuticle biosynthesis genes and cuticle properties. Reproduced from Kong et al. (2020), figure 6. Figure 1. Open in new tabDownload slide Schematic illustrating the proposed origins, evolutions, and characteristics of cuticle biosynthesis genes and cuticle properties. Reproduced from Kong et al. (2020), figure 6. Second, the very different life strategies of low-lying, moisture-loving bryophytes compared with taller vascular plants have led to different selective pressures and requirements for cuticle synthesis. It is possible that the high proportion of phenolics in bryophyte cuticles have a protective function against UV radiation and that strong hydrophobicity is less important for plants growing closer to water sources that can cope better with occasional desiccation. In fact, many bryophytes only produce significant cuticle quantities on certain surfaces such as sporophytes, more erect tissues, and around air pores, suggesting that cuticle is otherwise expendable (Schönherr and Ziegler, 1975; Budke and Goffinet, 2016; Renault et al., 2017). As P. patens showed a more lycophyte-like cuticle, it will be interesting to see whether this is a general feature of mosses and to understand why they may differ from other bryophytes. The plant cuticle was a pivotal feature allowing the success of plants on land. Diversification and expansion of the cuticle synthesis machinery probably contributed to the evolution of complex morphologies and size increases seen in seed plants. The systematic analysis provided by Kong et al. (2020) allows an in-depth understanding of cuticle synthesis, composition, and properties, highlighting the evolutionary pressures on cuticle formation and specialization within land plants. 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Plant Physiol 163 : 5 – 20 Google Scholar Crossref Search ADS PubMed WorldCat Author notes 1 Author for contact: maddy.seale@plants.ox.ac.uk. 2 Senior author. www.plantphysiol.org/cgi/doi/10.1104/pp.20.01445 © 2020 American Society of Plant Biologists. All Rights Reserved. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - The Fat of the Land: Cuticle Formation in Terrestrial Plants
JF - Plant Physiology
DO - 10.1104/pp.20.01445
DA - 2020-12-04
UR - https://www.deepdyve.com/lp/oxford-university-press/the-fat-of-the-land-cuticle-formation-in-terrestrial-plants-MhgM6LCEEH
SP - 1622
EP - 1624
VL - 184
IS - 4
DP - DeepDyve
ER -