TY - JOUR
AU - Chimote, V. P.
AB - Sorghum (Sorghum bicolor L.) is widely grown as a grain and forage crop and with its high biomass production has the potential to be a key biofuel crop. It is considered as a recalcitrant crop for in vitro regeneration. This study was conducted to optimize in vitro regeneration protocol and to evaluate the effects of plant growth regulators, proline and silver nitrate on in vitro callus and shoot growth differentiated from mature embryo and shoot tip explants in sweet, grain, and forage sorghum genotypes. Considerable variation in response to plant growth regulators, proline and silver nitrate was observed in all the genotypes. Among the four cytokinins used, zeatin was found to be better for in vitro shoot induction. Murashige and Skoog (MS) medium supplemented with proline and silver nitrate was shown to be superior to medium without proline and silver nitrate. The best callusing from mature embryos was observed on MS medium supplemented with 2.0 mg/l 2,4-dichlorophenoxyacetic acid (2,4-D), 2 mg/l silver nitrate, and 500 mg/l proline. Multiple shoot induction in shoot tip explants was higher in MS medium supplemented with 2.0 mg/l Zeatin, 2 mg/l silver nitrate, and 500 mg/l proline. Among the three genotypes, sweet sorghum genotype CSV 19 SS has the highest regeneration efficiency (92.8%). Proline and silver nitrate enhance the quality of callus and in vitro shoot regeneration in sorghum.
TI - Proline and Silver Nitrate Promotes Multiple Shoot Induction from Mature Embryo and Shoot Tip Explants of Sorghum
JF - Sugar Tech
DO - 10.1007/s12355-023-01277-w
DA - 2023-10-01
UR - https://www.deepdyve.com/lp/springer-journals/proline-and-silver-nitrate-promotes-multiple-shoot-induction-from-LuKEsdtRA3
SP - 1187
EP - 1195
VL - 25
IS - 5
DP - DeepDyve
ER -