TY - JOUR
AB -  The measurement of cell proliferation activity is one of the most important cell‐based assays and a cornerstone for high‐throughput discovery. Traditionally, one measures cell proliferation rates by counting the number of cells over a period of time. In contrast, Peacock and Wang have genetically engineered cells to produce green fluorescent protein (GFP) in a cell‐cycle dependent manner. Although transcription of the reporter gene occurs in pulses that coincide with the cell cycle, the authors were able to determine proliferation rates by normalizing the fluorescence intensity of GFP to that of a constitutively expressed red fluorescence protein. Because the fluorescent protein half‐lives were greater than cell doubling times, this normalized value was linearly proportional to the cell proliferation rate. This work also elucidates a “dilution” effect, where levels of long‐lived proteins can actually decrease with more frequent cell divisions, and illustrates the general importance of signal normalization for any genetic reporter system. Page 2003 
TI - A genetic tachometer for cell proliferation
JF - Biotechnology and Bioengineering
DO - 10.1002/bit.23236
DA - 2011-09-01
UR - https://www.deepdyve.com/lp/wiley/a-genetic-tachometer-for-cell-proliferation-LIXS1XEMb8
SP - FMVI
EP - FMVI
VL - 108
IS - 9
DP - DeepDyve
ER -