TY - JOUR
AU - KURUSU, Yasurou
AB - Abstract We describe the genetic analysis of the bio operon of the biotin auxotrophic Bacillus subtilis natto OK2 strain. The OK2 strain would only cross-feed with the Escherichia coli bioB mutant and also grew well in medium containing dethiobiotin. Sequencing analysis revealed two significant genetic alterations in the bioW and bioF genes within the bio operon of the OK2 strain. Complementation analysis with B. subtilis 168 bio mutants demonstrated that only the bioB gene could complement, but other bio operon genes could not. A bio+ transformant, isolated from an OK2 strain, has biotin autotrophy. Bacillus subtilis natto, biotin operon, bioB 1) Eisenberg, M. A., Biosynthesis of biotin and lipoic acid. In “Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology”, eds. Neidhardt, F. C., Ingraham, J. L., Magasanik, B., Low, K. B., Schaechter, M., and Umbarger, H. E., American Society for Microbiology, Washington, D.C., pp. 544–550 (1987). 2) Cronan, J. E. Jr., The E. coli bio operon: transcriptional repression by an essential protein modification enzyme. Cell, 58, 427–429 (1989). 3) Bower, S., Perkins, J. B., Rogers Yocum, R., Howitt, C. L., Rahaim, P., and Pero, J., Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon. J. Bacteriol., 178, 4122–4130 (1996). 4) Perkins, J. B., Bower, S., Howitt, C. L., Rogers Yocum, R., and Pero, J., Identification and characterization of transcripts from the biotin biosynthetic operon of Bacillus subtilis. J. Bacteriol., 178, 6361–6365 (1996). 5) Ploux, O., Soularue, P., Marquet, A., Gloeckler, R., and Lemoine, Y., Investigation of the first step of biotin biosynthesis in Bacillus sphaericus. Purification and characterization of the pimeloyl-CoA synthetase, and uptake of pimelate. Biochem. J., 287, 685–690 (1992). 6) Seki, T., and Ohshima, Y., Taxonomic position of B. subtilis. In “Bacillus subtilis: Molecular Biology and Industrial Application”, eds. Maruo, B., and Yoshikawa, H., Elsevier Science Publishers B.V., Amsterdam, pp. 7–25 (1989). 7) Ashikaga, S., Nanamiya, H., Ohashi, Y., and Kawamura, F., Natural genetic competence in Bacillus subtilis natto OK2. J. Bacteriol., 182, 2411–2415 (2000). 8) Hatakeyama, K., Kohama, K., Vertes, A. A., Kobayashi, M., Kurusu, Y., and Yukawa, H., Analysis of the biotin biosynthesis pathway in coryneform bacteria: cloning and sequencing of the bioB gene from Brevibacterium flavum. DNA Sequence, 4, 87–93 (1993). PDF This content is only available as a PDF. © 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © 2004 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
TI - Genetic Analysis of an Incomplete bio Operon in a Biotin Auxotrophic Strain of Bacillus subtilis Natto OK2
JF - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.68.739
DA - 2004-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/genetic-analysis-of-an-incomplete-bio-operon-in-a-biotin-auxotrophic-LBtpnSgzgL
SP - 739
EP - 742
VL - 68
IS - 3
DP - DeepDyve
ER -