TY - JOUR
AU - Scouten, W. H.
AB - Polymer‐shielded dye‐affinity chromatography is a form of chromatography in which the dye matrix forms complexes with a nonionic, water‐soluble polymer such as poly(vinylpyrrolidone) or poly(vinyl alcohol), prior to the column chromatography of a crude protein extract, the idea being that polymer shielding of the dye will prevent nonspecific interactions between the target protein and the dye. The concept of polymer shielding and a strategy for the rational selection of polymers suitable as shielding agents are presented.
TI - Polymer‐shielded dye‐affinity chromatography
JO - Journal of Molecular Recognition
DO - 10.1002/(SICI)1099-1352(199634/12)9:5/6<509::AID-JMR293>3.0.CO;2-Y
DA - 1996-10-01
UR - https://www.deepdyve.com/lp/wiley/polymer-shielded-dye-affinity-chromatography-Kkd7mlvnh3
SP - 509
EP - 514
VL - 9
IS - 5‐6
DP - DeepDyve
ER -