TY - JOUR
AU1 - TAKAGI, Hiroshi
AU2 - SUZUMURA, Akitoshi
AU3 - HASUURA, Yoshiyuki
AU4 - HOSHINO, Takayuki
AU5 - NAKAMORI, Shigeru
AB - Abstract An efficient procedure was established to select for thermostable proteases in an extreme thermophile, Thermus thermophilus. A non-protease-secreting mutant derived from T. thermophilus TH125 was used as host and the expression plasmid for aqualysin I from T. aquaticus YT-1 was constructed as a source of thermostable protease. T. thermophilus cells harboring the recombinant plasmid produced active aqualysin I into the medium and were able to grow on a minimal medium containing milk casein as the sole source of carbon and nitrogen. aqualysin I, screening, thermostable protease, Thermus thermophilus References 1) Kotsuka, T., Akanuma, S., Tomuro, M., Yamagishi, A., and Oshima, T., Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method. J. Bacteriol., 178, 723-727 (1996). 2) Akanuma, S., Yamagishi, A., Tanaka, N., and Oshima, T., Serial increase in the thermal stability of 3-isopropylmalate dehydrogenase from Bacillus subtilis by experimental evolution. Protein Sci., 7, 698-705 (1998). 3) Sakaki, Y. and Oshima, T., Isolation and characterization of a bacteriophage infectious to an extreme thermophile, Thermus thermophilus HB8. J. Virol., 15, 1449-1453 (1975). 4) Mather, M. W. and Fee, J. A., Development of plasmid cloning vectors for Thermus thermophilus HB8: expression of a heterologous, plasmid-borne kanamycin nucleotidyltransferase gene. Appl. Environ. Microbiol., 58, 421-425 (1992). 5) Maseda, H. and Hoshino, T., Development of expression vectors for Thermus thermophilus. J. Ferment. Bioeng., 86, 121-124 (1998). 6) Zhao, H., Giver, L., Shao, Z., Affholter, J. A., and Arnold, F. H., Molecular evolution by staggered extension process (StEP) in vitro recombination. Nature Biotech., 16, 258-261 (1998). 7) Zhao, H. and Arnold, F. H., Directed evolution converts subtilisin E into a functional equivalent of thermitase. Protein Eng., 12, 47-53 (1999). 8) Takagi, H., Suzumura, A., Hoshino, T., and Nakamori, S., Gene expression of Bacillus subtilis subtilisin E in Thermus thermophilus. J. Ind. Microbiol. Biotech., 23, 214-217 (1999). 9) Hidaka, Y., Hasegawa, M., Nakahara, T., and Hoshino, T., The entire population of Thermus thermophilus cells is always competent at any growth phase. Biosci. Biotechnol. Biochem., 58, 1338-1339 (1994). 10) Koyama, Y., Hoshino, T., and Tomizuka, N., Genetic transformation of the extreme thermophile Thermus thermophilus and other Thermus spp. J. Bacteriol., 166, 338-340 (1986). . 1992 . p. 143 - 149 . 12) Matsuzawa, H., Hamaoki, M., and Ohta, T., Production of thermophilic extracellular proteases (aqualysin I and II) by Thermus aquaticus YT-1, an extreme thermophile. Agric. Biol. Chem., 47, 25-28 (1983). 13) Matsuzawa, H., Tokugawa, K., Hamaoki, M., Mizoguchi, M., Taguchi, H., Terada, I., Kwon, S.-T., and Ohta, T., Purification and characterization of aqualysin I (a thermophilic alkaline serine protease) produced by Thermus aquaticus YT-1. Eur. J. Biochem., 171, 441-447 (1988). 14) Touhara, N., Taguchi, H., Koyama, Y., Ohta, T., and Matsuzawa, H., Production and extracellular secretion of aqualysin I (a thermophilic subtilisin-type protease) in a host-vector system for Thermus thermophilus. Appl. Environ. Microbiol., 57, 3385-3387 (1991). 15) Hoshino, T., Maseda, H., and Nakahara, T., Plasmid marker transformation in Thermus thermophilus. J. Ferment. Bioeng., 76, 276-279 (1993). 16) Venekei, I., Hedstrom, L., and Rutter, W. J., A rapid and effective procedure for screening protease mutants. Protein Eng., 9, 85-93 (1996). 17) Terada, I., Kwon, S.-T., Miyata, Y., Matsuzawa, H., and Ohta, T., Unique precursor structure of an extracellular protease, aqualysin I, with NH2- and COOH-terminal prosequences and its processing in Escherichia coli. J. Biol. Chem., 265, 6576-6581 (1990). 18) Maseda, H. and Hoshino, T., Screening and analysis of DNA fragments that show promoter activities in Thermus thermophilus. FEMS Microbiol. Lett., 128, 127-134 (1995). PDF This content is only available as a PDF. © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model) © 2000 by Japan Society for Bioscience, Biotechnology, and Agrochemistry
TI - Efficient Selection for Thermostable Protease in Thermus thermophilus
JF - Bioscience Biotechnology and Biochemistry
DO - 10.1271/bbb.64.899
DA - 2000-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/efficient-selection-for-thermostable-protease-in-thermus-thermophilus-KRr3S797Kd
SP - 899
EP - 902
VL - 64
IS - 4
DP - DeepDyve
ER -