TY - JOUR
AU - Urich,, Henry
AB - Abstract This immunohistochemical study compares the localization of the neuronal class III β-tubulin isotype (βIH) to that of calbindin-D28k in 40 human fetal and postnatal cerebella ranging from 12 weeks gestation to adulthood. In the external granule layer of the developing cerebellar cortex, βIII staining was present in the premigratory (postmitotic) zone of horizontal neurons but was absent in “epithelioid” cells of the subpial proliferative mitotic zone. In the molecular layer, intense βHI staining was associated with parallel fibers, stellate/basket neurons and migrating fusiform granule neurons. βIII staining was also present in internal granule neurons. In contrast, βIII was not detectable in fetal and neonatal Purkinje neurons and Golgi II neurons, but was evident in these neurons from juvenile and adult cerebella. Calbindin-D28k staining was present in Purkinje neurons also delineating their somatic spines (“pseudopodia”), lateralizing and apical dendrites (including dendritic spines), subpopulations of small to intermediate-sized Golgi II neurons in the internal granule layer (“synarmotic cells” of Landau), large to medium-sized subcortical Golgi II neurons and neurons of cerebellar roof nuclei, at various gestational stages and postnatally. It was absent in the external granule layer, parallel fibers, stellate/basket and internal granule neurons. Variable degrees of/3III and caIbindin-D28k staining were detected in subpopulations of immature neuroepithelial cells of the ventricular matrix at the roof of the fourth ventricle. Glial (including Bergmann glia) and mesenchymal cells were not stained for either antigenic determinants. The differential expression of calbindin-D28k and βIII defines distinct populations of neurons in the developing human cerebellar cortex and supports the ontogenetic concept of Ramon y Cajal. Calbindin-D28k, Cerebellum, Class HI β-tubulin isotype, External granule layer, Granule neuron, Purkinje neuron, Ventricular matrix This content is only available as a PDF. Author notes Parts of this work are included in the doctoral dissertation of CDK at the Laboratory of Descriptive Anatomy, Morphological and Functional Division, Athens Medical School, Athens, Greece (Director: Emeritus Professor Ioannis N. Vlachos). This work was presented, in part, at the XI International Congress of Neuropathology, Kyoto, Japan, September, 1990; the V Congress of the International Society of Greek Neuroscientists, Corfu, Greece, September, 1990; and the 67th Annual Meeting of the American Association of Neuropathologists, Baltimore, Maryland, June, 1991. Supported in part by the Singer Collaborative Grant and the Patrons of Research Fund at Beth Israel Medical Center (CDK); NIH NS21141 (AF) and NS20270 (SC). © 1993 by the American Association of Neuropathologists, Inc.
TI - Differential Localization of Class III β-Tubulin Isotype and Calbindin-D28k Defines Distinct Neuronal Types in the ...
JF - Journal of Neuropathology & Experimental Neurology
DO - 10.1097/00005072-199311000-00013
DA - 1993-11-01
UR - https://www.deepdyve.com/lp/oxford-university-press/differential-localization-of-class-iii-tubulin-isotype-and-calbindin-KGJSmlIYIn
SP - 655
EP - 666
VL - 52
IS - 6
DP - DeepDyve
ER -