TY - JOUR
AU1 - Shaik, Faruk Azam
AU2 - Lewuillon, Clara
AU3 - Guillemette, Aurélie
AU4 - Ahmadian, Bahram
AU5 - Brinster, Carine
AU6 - Quesnel, Bruno
AU7 - Collard, Dominique
AU8 - Touil, Yasmine
AU9 - Lemonnier, Loïc
AU1 - Tarhan, Mehmet Cagatay
AB - Analyzing cell–cell interaction is essential to investigate how immune cells function. Elegant designs have been demonstrated to study lymphocytes and their interaction partners. However, these devices have been targeting cells of similar dimensions. T lymphocytes are smaller, more deformable, and more sensitive to pressure than many cells. This work aims to fill the gap of a method for pairing cells with different dimensions. The developed method uses hydrodynamic flow focusing in the z-direction for on-site modulation of effective channel height to capture smaller cells as single cells. Due to immune cells' sensitivity to pressure, the proposed method provides a stable system without any change in flow conditions at the analysis area throughout experiments. Paired live cells have their activities analyzed with calcium imaging at the immunological synapse formed under a controlled environment. The method is demonstrated with primary human T lymphocytes, acute myeloid leukemia (AML) cell lines, and primary AML blasts.
TI - Pairing cells of different sizes in a microfluidic device for immunological synapse monitoring
JF - Lab on a Chip: Miniaturisation for Chemistry and Biology
DO - 10.1039/d1lc01156a
DA - 2022-01-26
UR - https://www.deepdyve.com/lp/royal-society-of-chemistry/pairing-cells-of-different-sizes-in-a-microfluidic-device-for-JsDjGaqIsx
SP - 908
EP - 920
VL - 22
IS - 5
DP - DeepDyve
ER -