TY - JOUR
AU - Peter, Matthias
AB - Many biological processes, such as development and cell cycle progression are tightly controlled by selective ubiquitin-dependent degradation of key substrates. In this pathway, the E3-ligase recognizes the substrate and targets it for degradation by the 26S proteasome. The SCF (Skp1–Cul1–F-box) and ECS (Elongin C–Cul2–SOCS box) complexes are two well-defined cullin-based E3-ligases
1,2,3
. The cullin subunits serve a scaffolding function and interact through their C terminus with the RING-finger-containing protein Hrt1/Roc1/Rbx1, and through their N terminus with Skp1 or Elongin C, respectively. In Caenorhabditis elegans, the ubiquitin-ligase activity of the CUL-3 complex is required for degradation of the microtubule-severing protein MEI-1/katanin at the meiosis-to-mitosis transition
4
. However, the molecular composition of this cullin-based E3-ligase is not known. Here we identified the BTB-containing protein MEL-26 as a component required for degradation of MEI-1 in vivo. Importantly, MEL-26 specifically interacts with CUL-3 and MEI-1 in vivo and in vitro, and displays properties of a substrate-specific adaptor. Our results suggest that BTB-containing proteins may generally function as substrate-specific adaptors in Cul3-based E3-ubiquitin ligases.
TI - The BTB protein MEL-26 is a substrate-specific adaptor of the CUL-3 ubiquitin-ligase
JO - Nature
DO - 10.1038/nature01959
DA - 2003-09-03
UR - https://www.deepdyve.com/lp/springer-journals/the-btb-protein-mel-26-is-a-substrate-specific-adaptor-of-the-cul-3-JeLgKBhShC
SP - 311
EP - 316
VL - 425
IS - 6955
DP - DeepDyve
ER -