TY - JOUR
AU1 - Shin, Seunghyeon
AU2 - Kim, Keunyoung
AU3 - Pak, Kyoungjune
AU4 - Nam, Hyun-Yeol
AU5 - Im, Hyung-Jun
AU6 - Lee, Myung Jun
AU7 - Kim, Seong-Jang
AU8 - Kim, In Joo
AB - ObjectivePositron emission tomography (PET) is a non-invasive technique measuring quantification of physiological and biochemical processes in the living organism. However, there are many considerations including anesthesia and fasting to acquire small animal imaging. We aimed to evaluate the effects of anesthesia and fasting of rats in dopamine transporter (DAT) imaging acquisition.MethodsMale Sprague Dawley (SD) rats aged 7 weeks and weighing 180–260 g were used in this study. Rats were randomly divided by 4 groups. Group A was kept under anesthesia for 40 min and fasted over 12 h. Group B was only fasted over 12 h. Group C was only kept under anesthesia for 40 min. Group D was neither kept under anesthesia nor fasted over 12 h. PET scans were started at 40 min after 18F-FP-CIT injection and obtained for 20 min. Volumes-of-interest for striatum and extrastriatal area were used for 18F-FP-CIT PET analysis. Cerebellum was considered as a reference region. Specific binding ratio (SBR) was calculated as follows: [(uptake of target-uptake of cerebellum)]/(uptake of cerebellum).ResultsSBR without fasting and anesthesia (group D) was significantly lower than those of other groups (vs group A, p = 0.0004; vs group B, p = 0.0377; vs group C, p = 0.0134). However, SBRs of extrastriatal area (p = 0.5120) were not affected by fasting and anesthesia.ConclusionsIn conclusion, the SBR of striatum was increased after anesthesia by isoflurane and fasting. When designing an experiment using DAT imaging, the effects of isoflurane and fasting should be considered.
TI - Effects of animal handling on striatal DAT availability in rats
JF - Annals of Nuclear Medicine
DO - 10.1007/s12149-020-01476-5
DA - 2020-07-01
UR - https://www.deepdyve.com/lp/springer-journals/effects-of-animal-handling-on-striatal-dat-availability-in-rats-JW4uEpWKED
SP - 496
EP - 501
VL - 34
IS - 7
DP - DeepDyve
ER -