TY - JOUR
AU - Carney, J, A
AB - Abstract We describe a radioassay for cobalamin (vitamin B12) in human serum or plasma that requires no boiling or other pretreatment of the sample. Normal chicken serum covalently coupled to magnetizable particles is used as the binding agent. The assay is performed at pH 12.9, at which pH all cobalamin in human serum is released from its binding proteins, whereas the binding agent maintains a high affinity for cobalamin (Ka 1.7 x 10(10) L/mol). Under these assay conditions the binding protein shows a specificity for cobalamin similar to that of purified intrinsic factor. The assay is simple, rapid, and precise, and results correlate well with those of the Euglena gracilis microbiological assay and an intrinsic-factor binding assay. This content is only available as a PDF. © 1981 The American Association of Clinical Chemistry This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Radioassay for cobalamin (vitamin B12) requiring no pretreatment of serum.
JO - Clinical Chemistry
DO - 10.1093/clinchem/27.2.263
DA - 1981-02-01
UR - https://www.deepdyve.com/lp/oxford-university-press/radioassay-for-cobalamin-vitamin-b12-requiring-no-pretreatment-of-JLYxMmdQu2
SP - 263
EP - 267
VL - 27
IS - 2
DP - DeepDyve
ER -