TY - JOUR
AU1 - Sampurno, Shienny
AU2 - Chittleborough, Timothy
AU3 - Carpinteri, Sandra
AU4 - Hiller, Jonathan
AU5 - Heriot, Alexander
AU6 - Lynch, Andrew
AU7 - Ramsay, Robert
AB - Background Insufflation with CO can employ continuous flow, recirculated gas and/or additional warming and humidifi- cation. The ability to compare these modes of delivery depends upon the assays employed and opportunities to minimize subject variation. The use of pigs to train colorectal surgeons provided an opportunity to compare three modes of C O delivery under controlled circumstances. Methods Sixteen pigs were subjected to rectal resection, insufflated with dry-cold CO (DC-CO ) (n = 5), recirculated CO by 2 2 2 an AirSeal device (n = 5) and humidic fi ation and warming (HW-CO2) by a HumiGard device ( n = 6). Peritoneal biopsies were harvested from the same region of the peritoneum for fixation for immunohistochemistry for hypoxia-inducible factor 1 alpha (HIF-1α) and scanning electron microscopy (SEM) to evaluate hypoxia induction or tissue/cellular damage, respectively. Results DC-CO insufflation by both modes leads to significant damage to mesothelial cells as measured by cellular bulg- ing and retraction as well as microvillus shortening compared with HW-CO at 1 to 1.5 h. DC-CO also leads to a rapid and 2 2 significant induction of HIF-1α compared with HW-CO . Conclusions DC-CO insufflation induces substantive cellular damage and hypoxia responses within the first hour 
TI - Modes of carbon dioxide delivery during laparoscopy generate distinct differences in peritoneal damage and hypoxia in a porcine model
JF - Surgical Endoscopy
DO - 10.1007/s00464-019-07213-y
DA - 2019-10-17
UR - https://www.deepdyve.com/lp/springer-journals/modes-of-carbon-dioxide-delivery-during-laparoscopy-generate-distinct-JK1iV1kVof
SP - 1
EP - 8
VL - OnlineFirst
IS - 
DP - DeepDyve
ER -