TY - JOUR
AU1 - Carimi, F.
AU2 - De Pasquale,  F.
AU3 - Crescimanno,  F. G.
AB - Callus induction, somatic embryogenesis and plant regeneration were obtained in six different citrus species ( Citrus deliciosa Ten. (cv 'Avana'), C . limon (L.) Burm. (cv 'Berna'), C . madurensis Lour. (cv 'CNR P9'), C . medica L. (cv 'Cedro di Trabia'), C . tardiva Hort. ex Tan. (cv 'CNR P6'), C . sinensis (L.) Osb. (cv 'Ugdulena 7')) from cultures of pistil transverse thin cell layer explants ((t)TCL). Explants were cultured on three different media: the nutrients and vitamins of Murashige and Skoog medium alone (MS) or MS supplemented with either 500 mg l –1 malt extract (MS I) or 500 mg l –1 malt extract and 13.3 μ M 6-benzylaminopurine (MS II). Sucrose (146 m M ) was used as the carbon source. Somatic embryos were visible 2–5 months after culture initiation. The different genotypes showed a different embryogenic frequency from stigma, style and ovary (t)TCL explants. All of the cultivars regenerated somatic embryos. Percentages of style (t)TCL explants producing somatic embryos ranged from 0% ( C . deliciosa , C . madurensis , C . sinensis and C . tardiva on the three different media) to 5.2% (C . limon on MS II). Embryo formation in stigma (t)TCL explants ranged from 0% ( C . madurensis on MS and MS I, C . sinensis on MS, C . deliciosa and C . tardiva on the three different media) to 42.4% ( C . limon on MS II). Embryo formation in ovary (t)TCL explants ranged from 0% ( C . deliciosa on MS, C . limon , C . medica , and C . sinensis on the three different media) to 9.3% ( C . tardiva on MS I). After about 12 weeks somatic embryos developed into plantlets at a high frequency.
TI - Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus
JF - Plant Cell Reports
DO - 10.1007/s002990050687
DA - 1999-08-01
UR - https://www.deepdyve.com/lp/springer-journals/somatic-embryogenesis-and-plant-regeneration-from-pistil-thin-cell-J7PzHpiDVu
SP - 935
EP - 940
VL - 18
IS - 11
DP - DeepDyve
ER -