TY - JOUR
AU1 - Zhang, Jing
AU2 - Yang, Junqing
AU3 - Luo, Ying
AU4 - Li, Jiansha
AU5 - Gong, Tao
AU6 - Huang, Suqiong
AU7 - Xiong, Yan
AU8 - Jiang, Xinhui
AB - Using 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate as a pre-column derivatization reagent, optimized derivatization and chromatography parameters, a simple high-performance liquid chromatography fluorescence detector (HPLC–FLD) method was developed and validated to determine 23 related amines in plasma and cortex of C57BL/6 mice with cerebral ischemia–reperfusion injury. The prepared samples were separated on a ZORBAX SB-C18 column (4.6 mm × 250 mm, 5 μm) with 60% acetonitrile (ACN) and 20 mM sodium acetate solution (pH adjusted to 5.0 by phosphoric acid). All analytes achieved good separation within 1.2 h at a flow rate of 1 mL/min. The limits of detection and limits of detection quantitation of the method were ranged from (0.1–9.2) to (0.3–30.6) ng/mL, respectively. The analytical method was apt for simultaneously determining 23 amino acids in plasma and cortex. Our results revealed that the relevant amino acids were significantly altered (P < 0.05) in C57BL/6 mice.
TI - Determination of 23 Amino Acids and Amines in Plasma and Cortex of C57BL/6 Mice with HPLC–FLD
JF - Journal of Chromatographic Science
DO - 10.1093/chromsci/bmac103
DA - 2022-12-26
UR - https://www.deepdyve.com/lp/oxford-university-press/determination-of-23-amino-acids-and-amines-in-plasma-and-cortex-of-IdvbTyTATN
SP - 44
EP - 57
VL - 62
IS - 1
DP - DeepDyve
ER -