TY - JOUR
AU - 
AB - HUNGARIAN JOURNAL OF INDUSTRY AND CHEMISTRY Vol. 46(1) pp. 13-17 (2018) hjic.mk.uni-pannon.hu DOI: 10.1515/hjic-2018-0004 CAPILLARY ISOTACHOPHORESIS DETERMINATION OF TRACE OXI- DIZED GLUTATHIONE IN BLOOD 1 2 2 1 RÓBERT BODOR , ANDREA NECASOVÁ , ALENA PECHOVÁ , AND MARIÁN MASÁR Department of Analytical Chemistry, Faculty of Natural Sciences, Comenius University in Bratislava, Ilkovico ˇ va 6, Bratislava, SK-84215, SLOVAK REPUBLIC Department of Animal Protection, Welfare and Behaviour, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences Brno, Palackého tˇ r. 1/3, Brno, CZ61242, CZECH REPUBLIC A capillary isotachophoresis (CITP) method performed in a column-coupling apparatus has been developed for the si- multaneous determination of glutathione (GSH) and glutathione disulfide (GSSG) concentrations in blood samples. The determination of GSSG and GSH concentrations in biological samples is important because of their roles in oxidative stress. Different concentrations of a leading ion in the coupled columns (concentration cascade) and a large volume (37 l) of the injected sample facilitated a GSSG concentration of between 2 and 25 mol/l. A reaction between iodoacetate and GSH under alkaline conditions was used to prepare the sample in order to avoid oxidation of GSH to GSSG. This step eliminated the main source of 
TI - Capillary Isotachophoresis Determination of Trace Oxidized Glutathione in Blood
JF - Hungarian Journal of Industry and Chemistry
DO - 10.1515/hjic-2018-0004
DA - 2018-07-01
UR - https://www.deepdyve.com/lp/unpaywall/capillary-isotachophoresis-determination-of-trace-oxidized-glutathione-I3giWfke5X
DP - DeepDyve
ER -