TY - JOUR
AU - Butler, M.
AB - Eight independent cell lines accumulated ammonia in culture to concentrations between 1.3 and 2.9 mM. The growth inhibition of such concentrations of ammonium chloride when added to culture medium was variable. The cell lines tested could be divided into 3 groups depending on their growth response to 2 mM added NH4CI. In the first group (293, HDF, Vero, and PQXB1/2) little (< 14%) or no growth inhibition occurred. In the second group (McCoy and MDCK) a reduction in final cell yield of 50-60% was observed. The third group (HeLa and BHK) was most sensitive to the effects of NH4CI with growth inhibition (>75%) compared to controls. The growth inhibitory effect of added lactate up to 20 mM was negligible (<10%) for 3 cell lines, although one cell line (PQXB1/2) showed greater sensitivity.
TI - Growth inhibition in animal cell culture
JF - Applied Biochemistry and Biotechnology
DO - 10.1007/BF02922022
DA - 2008-06-25
UR - https://www.deepdyve.com/lp/springer-journals/growth-inhibition-in-animal-cell-culture-HRvPU8qLET
SP - 29
EP - 41
VL - 30
IS - 1
DP - DeepDyve
ER -