TY - JOUR
AU - SPENCER, CAROLE, A.
AB - Since its introduction five years ago, the sensitive TSH (S-TSH) immunometric assay (IMA) has established itself as an important new thyroid test. In fact, TSH IMAs have now replaced the less sensitive first generation TSH radioimmunoassay (RIA) methods. As implied by the term sensitive, these TSH IMAs provide both improved analytic assay precision as well as clinical sensitivity for routinely distinguishing the suppressed serum TSH values characteristic of hyperthyroidism from euthyroidism (1). This improved sensitivity largely results from the IMA sandwich assay configuration in which two anti-TSH antibodies are employed, rather than the single antibody RIA methods (competitive ligand binding assays). Usually one or both of these antibodies are monoclonal and, therefore, possess a high degree of specificity for the TSH epitopes. The first antibody, which is present in excess and bound to a solid support such as a tube wall or bead, is directed at the specific β-subunit of TSH. This content is only available as a PDF. Author notes *This work was supported in part by NIH Grants DK-11727 and NCRR General Clinical Research Center Grant M01RR-43. Copyright © 1990 by The Endocrine Society
TI - The Use and Misuse of the Sensitive Thyrotropin Assays
JF - Journal of Clinical Endocrinology and Metabolism
DO - 10.1210/jcem-71-3-553
DA - 1990-09-01
UR - https://www.deepdyve.com/lp/oxford-university-press/the-use-and-misuse-of-the-sensitive-thyrotropin-assays-HMPV3tgR1b
SP - 553
EP - 558
VL - 71
IS - 3
DP - DeepDyve
ER -