TY - JOUR
AU - Gara,, Fergal
AB - Abstract A second adenylate cyclase (cya2) gene was isolated from a Rhizobium meliloti F34 gene bank. Complemented E. coli Acya mutants were capable of utilizing a number of, but not all, carbon sources known to be regulated by cAMP. DNA hybridization studies showed cya2 to be unique to R. meliloti strains. The cya2 nucleotide sequence was determined and found to encode a protein of 363 amino acids. Residues were identified within the C-terminal domain which are conserved in both eukaryotic adenylate and guanylate cyclases, including a putative ATP binding site. Similiar residues were also found in the prokaryotic R. meliloti Cya1 protein. A R. meliloti cyal/cya2 double mutant was constructed and characterized; however, cAMP production was still observed in this strain indicating the presence of a third cya gene. Cloning, Rhizobium meliloti, Adenylate cyclase, Sequence analysis, Eukaryotic References [1] Paigen K. Williams B. ( 1970 ) Catabolite repression and other control mechanisims in carbohydrate utilization Adv. Microb. Physiol. , 4 , 251 – 324 . Google Scholar Crossref Search ADS WorldCat [2] Guerinot M.L. Chelm B.K. ( 1984 ) Isolation and expression of the Bradyrhizobium japonicum adenylate cyalase gene (cya) in Echerichia coli J. Bacteriol. , 159 , 1068 – 1071 . Google Scholar PubMed OpenURL Placeholder Text WorldCat [3] Upchurch R.G. Elkan G.H. ( 1978 ) The role of ammonia l-glutamate and cAMP in the regulation of ammonia assimilation in Rhizobium japonicum Biochim. Biophys. Acta , 538 , 244 – 248 . Google Scholar Crossref Search ADS PubMed WorldCat [4] Lim S.T. Shanmugam K.T. ( 1979 ) Regulation of hydrogen utilization in Rhizobium japonicum by cyclic AMP Biochim. Biophys. Acta , 584 , 479 – 492 . Google Scholar Crossref Search ADS PubMed WorldCat [5] Wang Y.-P. Giblin L. Boesten B. O'Gara F. ( 1993 ) The Escherichia coli cAMP receptor protein (CRP) represses the Rhizobium meliloti dctA promoter in a cAMP dependant fashion Mol. Microbiol. , 8 , 253 – 259 . Google Scholar Crossref Search ADS PubMed WorldCat [6] Bianchini G.M. Carricarte V.C. Flawia M.M. Sanchez-Rivas C. ( 1993 ) Isolation of adenylate cyclase mutants from Rhizobium meliloti deficient in nodulation Wld. J. Microbiol. Biotec. , 9 , 168 – 173 . Google Scholar Crossref Search ADS WorldCat [7] Kiely B. O'Gara F. ( 1983 ) Cyclic 3′5′-adenosine monophosphate synthesis in Rhizobium: Identification of a cloned sequence from Rhizobium meliloti coding for adenylate cyclase Mol. Gen. Genet. , 192 , 230 – 234 . Google Scholar Crossref Search ADS WorldCat [8] O'regan M. Kiely B. O'gara F. ( 1989 ) Expression of the adenyl cyclase-encoding gene (cya) of Rhizobium meliloti F34: existence of two cya genes? Gene , 83 , 234 – 249 . Google Scholar Crossref Search ADS WorldCat [9] Hedegaard L. Danchin A. ( 1985 ) The cya gene region of Erwinia chrysanthemi B374: organisation and gene products Mol. Gen. Genet. , 201 , 38 – 42 . Google Scholar Crossref Search ADS WorldCat [10] Miller J.h. ( 1972 ) Experiments in Molecular Genetics Cold Spring Harbor Laboratory , Cold Spring Harbor, New York . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC [11] Abia H. Fujimoto S. Ozaki N. ( 1982 ) Molecular cloning and nucleotide sequencing of the gene for E. coli cAMP receptor protein Nucleic Acids Res. , 10 , 1345 – 1361 . Google Scholar Crossref Search ADS PubMed WorldCat [12] Bagdasarian M. Lurz R. Ruckert B. Franflin F.C.H. Bagdasarian M.M. Frey J. Timmis K.N. ( 1981 ) Specific purpose plasmid cloning II: Broad host range, high copy number RSF1010 derived vectors, and a host vector system for gene cloning in Pseudomonas Gene , 16 , 237 – 247 . Google Scholar Crossref Search ADS PubMed WorldCat [13] Wang Y.-P. Birkenhead K. Boesten B. Manian S.S. O'Gara F. ( 1989 ) Genetic analysis and regulation of the Rhizobium meliloti genes controlling C4-dicarboxylic acid transport Gene , 85 , 135 – 144 . Google Scholar Crossref Search ADS PubMed WorldCat [14] Simon R. Priefer U. Puhler A. ( 1983 ) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria Biotechnology , 784 – 791 . [15] Maniatis T. Fritsch E.F. Sambrook J. ( 1982 ) 2nd ed., In Molecular Cloning: A Laboratory Manual Cold Spring Harbor Laboratory , Cold Spring Harbor, NY . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC [16] Crasnier M. Dumay V. Danchin A. ( 1994 ) The catalytic domain of Escherichia coli K-12 adenylate cyclase as revealed by deletion analysis of the cya gene Mol. Gen. Genet. , 243 , 409 – 416 . Google Scholar Crossref Search ADS PubMed WorldCat [17] Lathigra R. O'Regan M. Kiely B. Boesten B. O'Gara F. ( 1986 ) Organization of the adenyl cyclase (cya) locus of Rhizobium meliloti Gene , 44 , 89 – 96 . Google Scholar Crossref Search ADS PubMed WorldCat [18] Renalier M.-H. Batut J. Ghai J. Terzaghi B. Gherardi M. David M. Garnerone A.-M. Vasse J. Truchet G. Huguet T. Boistard P. ( 1987 ) A new symbiotic cluster on the pSym megaplasmid of Rhizobium meliloti 2011 carries a functional fix gene repeat and a nod locus J. Bacteriol. , 169 , 2231 – 2238 . Google Scholar Crossref Search ADS PubMed WorldCat [19] Gottfert M. Horvath B. Kondorosi E. Putnoky P. Rodriguez-Quinones A. Kondorosi A. ( 1986 ) At least two nodD genes are necessary for efficient nodulation of alfalfa by R. meliloti Mol. Gen. Genet. , 128 , 789 – 798 . OpenURL Placeholder Text WorldCat [20] Chelm B.K. ( 1987 ) Developmental genetic and molecular biology of rhizobia-induced nodulation , 2nd ed. In Michigan State University, Department of Energy Plant Research Laboratory. 22nd Annual Report , InPlant Research, pp 29 – 38 Michigan State University , East Lansing . Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC [21] Beuve A. Boesten B. Crasnier M. Danchin A. O'Gara F. ( 1990 ) Rhizobium meliloti adenylate cyclase is related to eucaryotic adenylate and guanylate cyclases J. Bacteriol. , 172 , 2614 – 2621 . Google Scholar Crossref Search ADS PubMed WorldCat [22] Beuve A. Kirn E. Danchin A. ( 1993 ) Rhizobium meliloti adenylate cyclase: probing of a NTP-binding site common to cation transporters C.R. Acad. Sci. Paris , 316 , 553 – 559 . OpenURL Placeholder Text WorldCat [23] Higgins C.F. Hiles I.D. Salmond G.P.C. Gill D.R. Downie J.A. Evans I.J. Holland I.J. Gray L. Buckel S.D. Bell A.W. Hermodson M.A. ( 1986 ) A family of related ATP-binding subunits coupled to many distinct biological processes in bacteria Nature , 323 , 449 – 450 . Google Scholar Crossref Search ADS WorldCat [24] Peterkofsky A. Reizer A. Reizer J. Gollop N. Zhu P.-P. Amin N. ( 1993 ) Bacterial adenylyl cycalses Prog. Nucleic Acids Res. Mol. Biol. , 44 , 31 – 65 . Google Scholar Crossref Search ADS WorldCat [25] Dayhoff M.O. ( 1978 ) Atlas of Protein Sequence and Structure , pp 1 – 8 National Biomedical , USA Chapter 5. Google Scholar Google Preview OpenURL Placeholder Text WorldCat COPAC This content is only available as a PDF. Author notes 1 Schering Plough, Brinny, Co. Cork, Ireland. © 1995 Federation of European Microbiological Societies
TI - Cloning of the second adenylate cyclase gene (cya2) from Rhizobium meliloti F34: Sequence similarity to eukaryotic cyclases
JF - FEMS Microbiology Letters
DO - 10.1111/j.1574-6968.1995.tb07519.x
DA - 1995-05-01
UR - https://www.deepdyve.com/lp/oxford-university-press/cloning-of-the-second-adenylate-cyclase-gene-cya2-from-rhizobium-HIZ125POSl
SP - 177
EP - 184
VL - 128
IS - 2
DP - DeepDyve
ER -