TY - JOUR AU - N. Bossak, Hilfred AB - AN APPARATUS FOR TH E ASEPTIC COLLECTION AND DISPENSING OF SHEEP BLOOD* HILPRE D N. BOSSAK, B.S. From the Venereal Disease Research Laboratory, U. S. Public Health Service, Staten Island, New York An apparatus for collecting and dispensing sheep blood aseptically has been used at the Veneral Disease Research Laboratory for several years with satis­ factory results. The assembly as herein described allows the collection, filtration and dispensing of the blood from a single unit, thereby reducing the opportunities for bacterial contamination. Any suitable preservative may be employed with this apparatus. A simple and reliable method for preserving sheep cells with 3.8 per cent so­ dium citrate solution, for use in complement-fixation procedures, has been pre­ viously reported. Sheep blood preserved by this method, and collected, dis­ pensed and stored as described below remained sterile and satisfactory for use for a three-month period. EQUIPMEN T 1. Flask, 2 liter Erlenmeyer, for collecting blood. 2. Bleeding apparatus consisting of a #10 2-hole rubber stopper, filter funnel (1 inch in diameter, 2 | inch stem),f wire mesh thimble, gauze, rubber and glass tubing, and a 13 gauge hypodermic needle (Fig. 1A). 3. Dispensing assembly consisting of rubber tubing, clamp and filling TI - An Apparatus For the Aseptic Collection and Dispensing of Sheep Blood* JF - American Journal of Clinical Pathology DO - 10.1093/ajcp/19.5_ts.496 DA - 1949-05-01 UR - https://www.deepdyve.com/lp/oxford-university-press/an-apparatus-for-the-aseptic-collection-and-dispensing-of-sheep-blood-GOTfh2drxG SP - 496 EP - 498 VL - 19 IS - 5_ts DP - DeepDyve ER -