TY - JOUR
AU - SHIMOKORIYAMA,, Masami
AB - Abstract Cytochrome c-552 was extracted from a red alga, Polysiphonia urceolata, by immersion of the frozen trichomes in deionized water. Purification was carried out by acrinol treatment, ammonium sulfate fractionation, DEAE-Sephacel chromatography, hydroxyapatite column chromatography, and Bio-Gel P-10 gel filtration. The ferrocytochrome c-552 has absorption maxima at 551.5(α), 522.5(β), 416.3(γ), 318(δ), 292, and 270 nm; those of the ferricytochrome are at 525, 408.5(γ), and 358 nm. The pyridine ferrohemochrome showed absorption maxima at 550(α), 520(β), and 414 nm(γ). The α-band of the ferrocytochrome is symmetric without any shoulder at room temperature, and does not split even at liquid nitrogen temperature. The ferricytochrome showed a weak absorption shoulder at 695 nm, suggesting a methionine sulfur to be the sixth ligand of heme ciron. The cytochrome is oxidized by ferricyanide and reduced by ferrocyanide, cysteine, ascorbate, and hydrosulfite. Autoxidation was not observed. The midpoint potential (Em) of the cytochrome was determined by equilibration with the ferro and ferricyanide system to be 0.333 volt at pH 7.0 and 25°C. The isoelectric points of the ferro and ferricytochromes were determined to be at pH3.85 and 4.02, respectively, by the isoelectric focusing method. The molecular weight was estimated to be about 12,000 from the results of gel filtration and SDS polyacrylamide gel electrophoresis. This content is only available as a PDF. Author notes 1This work was supported in part by a Grant-in-Aid for Special Project Research (No. 58112004) from the Ministry of Education, Sciecne and Culture of Japan. 2Present address: Junsei Chemical Co., Ltd., Koshigaya, Saitama 343. © 1984, by the Japanese Biochemical Society
TI - Studies on Algal Cytochromes. V. Purification and Characterization of Cytochrome c-552 from a Red Alga, Polysiphonia urceolata
JF - The Journal of Biochemistry
DO - 10.1093/oxfordjournals.jbchem.a135000
DA - 1984-10-01
UR - https://www.deepdyve.com/lp/oxford-university-press/studies-on-algal-cytochromes-v-purification-and-characterization-of-GNrPXGpWvR
SP - 1681
EP - 1687
VL - 96
IS - 6
DP - DeepDyve
ER -