TY - JOUR
AU1 - Linxweiler, Johannes
AU2 - Hammer, Markus
AU3 - Muhs, Stefanie
AU4 - Kohn, Moritz
AU5 - Pryalukhin, Alexej
AU6 - Veith, Christian
AU7 - Bohle, Rainer
AU8 - Stöckle, Michael
AU9 - Junker, Kerstin
AU1 - Saar, Matthias
AB - Purpose To generate and characterize 3D spheroid suspension cultures from radical prostatectomy (RP) specimens as a versatile model system for organ-confined prostate cancer (PCa). Methods Cancerous tissue samples from RP specimens were excised by a uropathologist. Preparation of 3D spheroids was done by mechanical disintegration and limited enzymatic digestion followed by serial filtration through 100 μm- and 40 μm-cell strainers. Thereafter, spheroids were cultured in a modified stem cell medium and characterized by a live/dead assay, whole-spheroid immunohistochemistry (IHC; CK5, CK8, AMACR, PSA, Ki67, AR, αSMA, Vimentin, E-Cadherin) and PSA-measurements in culture medium. Furthermore, their response to pharmaceutical treatment with docetaxel, bicalu- tamide, enzalutamide and abiraterone was tested. Results 173 RP cases were included. The median preoperative PSA-level was 16.12 ng/ml [range 0.99;345], the median Gleason score was 7b [6;10]. 64 cases were excluded due to low tumor content in frozen sections (43) or to insufficient sphe- roid formation (21). In the remaining 109 cases, spheroids formed successfully and stayed viable for up to several months. IHC analysis revealed AR-, CK8-, and AMACR-positivity in nearly all cases, while CK5-positive cells were detectable only occasionally as were α-SMA and Vimentin. E-Cadherin was positive in most cases. Furthermore, spheroids proved to be amenable to 
TI - Patient-derived, three-dimensional spheroid cultures provide a versatile translational model for the study of organ-confined prostate cancer
JF - Journal of Cancer Research and Clinical Oncology
DO - 10.1007/s00432-018-2803-5
DA - 2018-11-24
UR - https://www.deepdyve.com/lp/springer-journals/patient-derived-three-dimensional-spheroid-cultures-provide-a-GLpXZp9Uxe
SP - 551
EP - 559
VL - 145
IS - 3
DP - DeepDyve
ER -