TY - JOUR AB - Introduction Materials and Methode In a previous report (1) we described the preparation and the stability of some ethylene glycol-stabilized haemolysates, which can be used äs control matenals for the glycated haemoglobin assay. The haemolysates were prepared from normal and diaJ. Clin. Chem. Clin. Biochem. / Vol. 25,1987 / No. 7 HbAi and HbA ic measurements The affinity Chromatographie method commercialized by Pierce (GlycpTest, Pierce Eurochemie PV, Ba, Holland) was performed at 23 °C, fpllowing the directions of the manufacturer. A new semi-automated High-Performance Liquid Chromatographie (HPLC) method, introduced by Bio-Rad Laboratories (Milan), was also used. The method is based on an apparatus (HLC-723GHb Glycopack-Diamat) which we had the opportunity to evaluate in our laboratory (2). This System also works at 23 °C, and is able to separate, in order of elution from the column, the following minor haemoglobin fractions: HbAJa, HbAlb, HbF, HbAlc and HbA0. The other measurements (HbAj and HbAlc minicolumns, spectrophotometry) were performed äs described (1). Inter-laboratory trial on the determination pf glycated haemoglobins An inter-laboratory Quality Control programme among 15 Italian laboratories was performed, using two lots (a low and a high HbAlc level) of the ethylene-glycol-stabilized haemolysates, prepared and sent express to the TI - SHORT COMMUNICATION/KURZMITTEILUNG JO - Clinical Chemistry and Laboratory Medicine DO - 10.1515/cclm.1987.25.7.447 DA - 1987-01-01 UR - https://www.deepdyve.com/lp/de-gruyter/short-communication-kurzmitteilung-GI0FC1jA0Q SP - 447 EP - 450 VL - 25 IS - 7 DP - DeepDyve ER -