TY - JOUR AU - S. Kayashima , T. Arai , M. Kikuchi , N. Nagata , N. Ito , T. Kuriyama , and J. Kimura AB - MATERIALS AND METHODS apparatus. Because the SEF is a small-volume sample and the sampling is difficult to handle, the authors devised a novel apparatus. After albino Japan White rabbits (2.0-2.3 kg/body wt, female; Tokyo Laboratory Animal Science, Tokyo, Japan) were prepared (see SEF sampling method), the apparatus was attached. Figure IA shows a side view of the apparatus, used for measuring SEF rate and analyzing its constituents. It consisted of a 3-cm-diameter diskshaped apparatus attached to a graduated reservoir. Its weight was -20 g, light enough to be easily fixed to the skin, and the area which contacted the skin was smoothly polished to prevent skin irritation. The upper portion of the apparatus was connected to a decompressing pump that ed the skin at 400 mmHg absolute pressure. This pressure was selected for the procedure not only to avoid possible damage to the skin surface by the but also to maintain a higher SEF rate. A stainless steel mesh was inserted between the skin and the apparatus to maintain an effective area (7.1 cm’)). The SEF rate was measured during for 4 h, and accumulated SEF was subjected to constituent analysis. Figure 1B shows a side view of TI - Suction effusion fluid from skin and constituent analysis: new candidate for interstitial fluid JF - AJP - Heart and Circulatory Physiology DA - 1992-11-01 UR - https://www.deepdyve.com/lp/the-american-physiological-society/suction-effusion-fluid-from-skin-and-constituent-analysis-new-G0oRj89aRW VL - 263 IS - DP - DeepDyve ER -