TY - JOUR
AU - Taniguchi, Satoshi
AB - Abstract We examined the effects of interleukin 3 (IL-3), kit ligand and erythropoietin (EPO) on the survival and growth of early appearing spleen colony forming units (CFU-S8) and late appearing CFU-S (CFU-S12) in short-term liquid culture (SLC). In the control cultures, without any additive, CFU-S8 and CFU-S,2 declined; nearly 10% of the initial number of CFU-S still survived by the second day of culture. The addition of IL-3 or kit.-ligand increased the survival both of CFU-S, and CFU-S12, with an increased dose of concentration, at final concentrations of 10-200 U/ml and 500-50 dilution, respectively. However, only the survival of CFU-S, increased when EPO was added up to 10 U/ml, while the frequency of CFU-S12 was not higher than in the control culture. The percentage of CFU-S8 and CFU-S12 in DNA synthesis, evaluated in 3H-TdR cytocide experiments, increased after one day in culture with each of the three factors. 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Google Scholar Crossref Search ADS PubMed WorldCat This content is only available as a PDF. © 1993 AlphaMed Press This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model)
TI - Promotion of survival and proliferation by interleukin 3, fcil-ligand and erythropoietin on early and late appearing spleen colony forming units in culture
JF - Stem Cells
DO - 10.1002/stem.5530110625
DA - 1993-01-01
UR - https://www.deepdyve.com/lp/oxford-university-press/promotion-of-survival-and-proliferation-by-interleukin-3-fcil-ligand-G01Ojg0WhU
SP - 555
EP - 561
VL - 11
IS - 6
DP - DeepDyve
ER -