TY - JOUR
AU - 
AB - Here we report the reaction between biotin and azide-labelled oxaziridine reagents in aqueous conditions at room temperature. This method, which we call biotin redox-activated chemical tagging (BioReACT), achieves efficient and stable labelling of proteins with oxaziridine reagents. We functionally validate the method by generating an antibody-drug conjugate and numerous flow-cytometry reagents. Finally, we conjugate a functional click handle to a biotinylated oligonucleotide. These studies show that the biotin–oxaziridine reaction is a powerful approach for the efficient synthesis of stable protein and DNA bioconjugates.
TI - Biotin as a Reactive Handle to Selectively Label Proteins and DNA with Small Molecules
JF - ChemRxiv
DO - 10.26434/chemrxiv.13524296.v1
DA - 2021-01-06
UR - https://www.deepdyve.com/lp/chemrxiv/biotin-as-a-reactive-handle-to-selectively-label-proteins-and-dna-with-Fv0wduRSlc
DP - DeepDyve
ER -