TY - JOUR
AU - Zhang, Si-Liang
AB - Previous research has confirmed that cobalt ion and dimethylbenzimidazole (DMBI) are the precursors of vitamin B12 biosynthesis, and porphobilinogen synthase (PBG synthase) is a zinc-requiring enzyme. In this paper, the effects of Zn2+, Co2+ and DMBI on vitamin B12 production by Pseudomonas denitrificans in shake flasks were studied. Present experimental results demonstrated that the addition of the above mentioned three components to the fermentation medium could significantly stimulate the biosynthesis of vitamin B12. The concentrations of zinc sulphate, cobaltous chloride and DMBI in the fermentation medium were further optimized with rotatable orthogonal central composite design and statistical analysis by Data Processing System (DPS) software. As a result, vitamin B12 production was increased from 69.36 ± 0.66 to 78.23 ± 0.92 μg/ml.
TI - Influence of Zn2+, Co2+ and dimethylbenzimidazole on vitamin B12 biosynthesis by Pseudomonas denitrificans
JO - World Journal of Microbiology and Biotechnology
DO - 10.1007/s11274-008-9770-y
DA - 2008-05-26
UR - https://www.deepdyve.com/lp/springer-journals/influence-of-zn2-co2-and-dimethylbenzimidazole-on-vitamin-b12-Fbp8hkv0iO
SP - 2525
EP - 2530
VL - 24
IS - 11
DP - DeepDyve
ER -